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Fc受体蛋白

治疗性抗体的功效不仅取决于其Fab片段结合靶抗原的活性,还取决于其Fc片段与Fc受体的相互作用。Fc片段与FcRn(FCGRT&B2M)受体的亲和力可以预测抗体的半衰期,而Fc片段和FcγRⅢA(CD16a)的结合会影响抗体依赖的细胞介导的细胞毒性作用(Antibody-Dependent Cell-mediated Cytotoxicity)。因此,优化抗体结构,筛选获得和Fc受体亲和力最理想的抗体,是发展治疗性单克隆抗体的一个重要途径。

ACROBiosystems公司提供一系列重组Fc受体蛋白,包括了常见的突变体,以帮助加速单克隆抗体的开发。同时,基于我司丰富的蛋白产品资源和活性分析经验,您只需提供样品,我们即可为您提供优质SPR&BLI亲和力检测服务,且免费提供实验所需所有高质量Fc受体蛋白!点击快速咨询>>>

产品特点

高纯度

为了满足抗体药研发过程中对用于检测和筛选的蛋白质纯度高的需求,我们的QC团队对大多数重组蛋白都同时进行了SDS-PAGE和HPLC分析。值得注意的是,Fc受体的单体形式反映了其天然状态,应该使用单体纯度尽可能高的Fc受体来验证和抗体的亲和力,以避免因聚集效应而产生的结合活性虚高现象。因此,我们通过HPLC检测建立了严格的单体纯度内部控制标准,只有满足标准的批次才会被放行。

FCM-H5286-HPLC
图1. HPLC分析,Human Fc gamma RIIA / CD32a ( R167 ) Protein ( Cat. No. CDA-H5221 ) 纯度大于95%。

亲和力经SPR&BLI验证

分子间的相互作用可以通过多种方法检测。传统的ELISA方法不适合检测像CD16和CD32这样的低亲和力受体与抗体的相互作用,而SPR则成为研究Fc受体与抗体亲和力最常用的方法。为了保证我们的Fc受体能够达到预期的性能,我们的QC团队使用Biacore平台对我们大部分产品进行测试。此外,为了满足客户在抗体药研发过程中不同的应用场景,我们同样采用BLI技术进行了亲和力的验证。所有展现在网页上的SPR&BLI数据都免费提供内部实验操作方法供参考

FCA-H52H2-S1.jpg
图2. SPR验证,固定在CM5芯片上的Human CD64, His Tag (Cat. No. FCA-H52H1) 可以结合Herceptin ( 赫赛汀单抗 ),亲和常数为4.92 nM ( Biacore 8K )。
申请SPR Protocol
FCM-H5286-S1
图3. SPR验证,将Human FCGRT&B2M Heterodimer Protein (Cat. No. FCM-H5286)通过anti-His抗体固定在CM5芯片上,可以结合Herceptin (赫赛汀单抗),亲和常数为0.261 μM (Biacore T200)。
申请SPR Protocol
FCA-H52H1-B1.jpg
图4. BLI验证,将Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H1)固定在HIS1K生物传感器上,可以结合Herceptin (赫赛汀单抗),亲和常数为6.98 nM (ForteBio Octet Red96e)。
申请BLI Protocol
FCN-H52W7-B1
图5. BLI验证,将Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) (Cat. No. FCN-H52W7)通过生物素化的his antibody 固定在SA传感器上,可以结合Herceptin (赫赛汀单抗),亲和常数为0.14 μM (ForteBio Octet Red96e)。
申请BLI Protocol

高批间一致性

我们通过采取严格的质量控制措施,以确保我们产品的批间一致性。如下图所示,被测样品的批间差异很小。

FCM-H82W4-E2
图6. SPR验证,固定在CM5芯片上的Human Fc gamma RIIB / CD32b Protein (Cat. No. CDB-H5228)可以结合Rituximab (利妥昔单抗),亲和常数为10μM (Biacore T200)。
申请SPR Protocol

用相似度评分软件分析可以看到,不同批次的CD32b相似度非常高,体现了高批间一致性。

FCM-H82W4-E2
图7. 不同批次的Human Fc gamma RIIB / CD32b ( Cat. No. CDB-H5228 ) 相似度在90%以上。

生物素标记或其他种属的Fc受体蛋白

ACROBiosystems提供即时可用的生物素标记Fc受体蛋白,这些产品具有高活性、高批间一致性等特点,为抗体药研发提供更多的选择。此外,我们还提供如猴、小鼠、大鼠等其他种属的Fc受体蛋白,用于筛选非人源化的抗体或验证种属交叉反应。

CDA-H82E9-S1
图8. SPR验证,通过生物素捕获固定在Biotin CAP- Series S Sensor芯片上的Biotinylated Human CD16a ( V176 ), His Tag, Avi Tag ( Cat. No. CDA-H82E9 ) 可以结合Rituximab ( 利妥昔单抗 ),亲和常数为0.261 μM ( Biacore T200 )。
申请SPR Protocol
FCM-C5284-S1
图9. SPR验证,固定在CM5芯片上的Cynomolgus / Rhesus macaque FcRn Protein ( Cat. No. FCM-C5284 ) 可以结合Rituximab ( 利妥昔单抗 ),亲和常数为0.403 μM ( Biacore T200 )。
申请SPR Protocol

产品列表

分子 货号 host 产品描述 结构
CD23CD3-H82Q5HEK293Biotinylated Human CD23 / Fc epsilon RII Protein, His,Avitag™
CD3-H5249HEK293Human CD23 / Fc epsilon RII Protein, His Tag
Fc gamma RI / CD64FCA-H52H1HEK293Human Fc gamma RI / CD64 Protein, His Tag (SPR & BLI verified)
FCA-C82E8HEK293Biotinylated Cynomolgus Fc gamma RI / CD64 Protein, His,Avitag™ (BLI verified)
CD4-M5227HEK293Mouse Fc gamma RI / CD64 Protein, His Tag (BLI verified)
FCA-H82E8HEK293Biotinylated Human Fc gamma RI / CD64 Protein, His,Avitag™ (SPR & BLI & HPLC verified)
Fc gamma RIIA / CD32aCDA-H82E6HEK293Biotinylated Human Fc gamma RIIA / CD32a (H167) Protein, Avitag™,His Tag (SPR & BLI verified)
CDA-H82E7HEK293Biotinylated Human Fc gamma RIIA / CD32a (R167) Protein, Avitag™,His Tag (SPR & BLI verified)
CDA-H5221HEK293Human Fc gamma RIIA / CD32a (R167) Protein, His Tag (BLI verified)
CD1-H5223HEK293Human Fc gamma RIIA / CD32a (H167) Protein, His Tag (SPR & BLI verified)
CDA-C82E5HEK293Biotinylated Cynomolgus Fc gamma RIIA / CD32a Protein, His,Avitag™ (SPR & BLI verified)
CDA-C52H7HEK293Cynomolgus Fc gamma RIIA / CD32a Protein, His Tag (SPR & BLI & HPLC verified)
CDA-R52H4HEK293Rhesus macaque Fc gamma RIIA / CD32a Protein, His Tag (SPR & BLI verified)
Fc gamma RIIB / CD32bCDB-C82E4HEK293Biotinylated Cynomolgus Fc gamma RIIB / CD32b Protein, His,Avitag™ (SPR & BLI verified)
CDB-H5228HEK293Human Fc gamma RIIB / CD32b Protein, His Tag (SPR & BLI verified)
CDB-M82E8HEK293Biotinylated Mouse Fc gamma RIIB / CD32b Protein, Avitag™,His Tag (SPR & BLI verified)
CDB-H82E0HEK293Biotinylated Human Fc gamma RIIB / CD32b Protein, Avitag™,His Tag (SPR & BLI verified)
CDB-M52H7HEK293Mouse Fc gamma RIIB / CD32b Protein, His Tag (SPR & BLI verified)
Fc gamma RIII / CD16CDA-M52H8HEK293Mouse Fc gamma RIII / CD16 Protein, His Tag (SPR & BLI verified)
FC6-C52H9HEK293Cynomolgus Fc gamma RIII / CD16 Protein, His Tag (BLI verified)
FC6-C82E0HEK293Biotinylated Cynomolgus Fc gamma RIII / CD16 Protein, His,Avitag™ (BLI verified)
FC6-R52H6HEK293Rhesus macaque Fc gamma RIII / CD16 Protein, His Tag (SPR & BLI verified)
Fc gamma RIIIA / CD16aCDA-H82E8HEK293Biotinylated Human Fc gamma RIIIA / CD16a (F176) Protein, Avitag™,His Tag (SPR & BLI verified)
CDA-H82E9HEK293Biotinylated Human Fc gamma RIIIA / CD16a (V176) Protein, Avitag™,His Tag (SPR & BLI verified)
CDA-H5220HEK293Human Fc gamma RIIIA / CD16a (F176) Protein, His Tag (SPR & BLI verified)
CD8-H52H4HEK293Human Fc gamma RIIIA / CD16a (V176) Protein, His Tag (SPR & BLI verified)
Fc gamma RIIIB / CD16b (NA1)CDB-H5227HEK293Human Fc gamma RIIIB / CD16b (NA1) Protein, His Tag (SPR & BLI verified)
CDB-H82E4HEK293Biotinylated Human Fc gamma RIIIB / CD16b (NA1) Protein, His,Avitag™ (SPR & BLI verified)
Fc gamma RIIIB / CD16b (NA2)CDB-H5222HEK293Human Fc gamma RIIIB / CD16b (NA2) Protein, His Tag (SPR & BLI verified)
CDB-H82EaHEK293Biotinylated Human Fc gamma RIIIB / CD16b (NA2) Protein, His,Avitag™ (SPR & BLI verified)
Fc gamma RIV / CD16-2FC4-M52H3HEK293Mouse Fc gamma RIV / CD16-2 Protein, His Tag
FC4-M82E8HEK293Biotinylated Mouse Fc gamma RIV / CD16-2 Protein, His,Avitag™ (BLI verified)
FcRn (FCGRT & B2M)FCM-C82W5HEK293Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag™&Strep II Tag (BLI verified)
FCM-H82W4HEK293Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, Avitag™,His Tag&Strep II Tag (SPR & BLI verified)
FCM-H8286HEK293Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, ultra sensitivity (primary amine labeling) (SPR verified)
FCM-M82W6HEK293Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, Avitag™,His Tag&Twin-Strep Tag (BLI verified)
FCM-R82W7HEK293Biotinylated Rat FcRn / FCGRT&B2M Heterodimer Protein, Avitag™,His Tag&Strep II Tag (SPR verified)
FCM-C5284HEK293Cynomolgus / Rhesus macaque FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (BLI verified)
FCM-M82W5HEK293Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag™ (BLI verified)
FCM-H5286HEK293Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified)
FCM-M52W2HEK293Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (BLI verified)
FCM-P5280HEK293Porcine FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified)
FCM-R5287HEK293Rat FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified)
FCN-F82W3HEK293Biotinylated Feline FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag™ (SPR verified)
FCM-C82W3HEK293Biotinylated Cynomolgus / Rhesus macaque FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag™ (SPR verified)
FCN-B82W3HEK293Biotinylated Bovine FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag™ (SPR verified)
FCM-M52W8HEK293Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag (BLI verified)
FCN-H52W7HEK293Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified)
FCM-C52W9HEK293Cynomolgus / Rhesus macaque FcRn / FCGRT&B2M Heterodimer Protein, His Tag (BLI verified)
FCM-H82W7HEK293Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag™ (SPR verified)
FCM-R52W5HEK293Rabbit FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (SPR verified)

部分引用文献

Single chain Fc-dimer-human growth hormone fusion protein for improved drug delivery.

Authors: Li Zhou et al.

Journal: Biomaterials 2017

Product: Human FcRn(Cat. No.  FCM-H5286

Application: SPR

Download PDF: Please click to download the PDF

Charge variant analysis of proposed biosimilar to Trastuzumab

Structural basis for pH-insensitive inhibition of immunoglobulin G recycling by an anti-neonatal Fc receptor antibody

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