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高亲和力CD19蛋白,专为检测CD19-CAR-T而设计

据ClinicalTrial.gov的数据显示,截至今年7月,全球登记开展的CAR-T临床研究项目达580余项。其中,CD19-CAR-T临床研究项目达170余项,占到总数近30%,是CAR-T临床研究中最热门的靶点。

对于CAR-T细胞来说,发挥肿瘤杀伤作用的有效成分是CAR阳性的T细胞,CAR-T细胞产品的包装规格及临床使用剂量是以CAR-T阳性细胞数表示的,因此,CAR转染阳性率是CAR-T质量控制的必检项,监管部门建议应采用流式细胞法检测CAR转染阳性率。目前有针对CAR不同结构区域的检测方法,包括针对CAR抗原结合位点的,比如CD19抗原,或针对轻链或铰链区的抗Fab抗体或Protein L蛋白,其中,针对抗原结合部位的CAR阳性率检测方法因其具有更好专属性而被广泛使用。

尽管CD19蛋白是检测CD19-CAR阳性率最适合的工具,但市面上却很难找到能够高效检测CD19-CAR-T的CD19蛋白。ACROBiosystems为解决这个问题,专门设计开发出了一系列包括非标记、生物素标记、荧光标记等多种形式的高亲和力CD19蛋白,并通过各种检测方法对其性能进行了全方位的分析。

产品列表

分类 货号 产品描述
荧光标记CD19 CD9-HF2H2 FITC-Labeled Human CD19 (20-291) Protein
CD9-HF251 FITC-Labeled Human CD19 (20-291) Protein, Fc Tag
生物素标记CD19 CD9-H8259 Biotinylated Human CD19, Fc Tag, ultra sensitivity (primary amine labeling)
非标记CD19 CD9-H52H2 Human CD19 (20-291) Protein, His Tag
CD9-H5251 Human CD19 (20-291) Protein, Fc Tag, low endotoxin (Super affinity)
CD9-H5250 Human CD19 (20-291) Protein, Llama IgG2b Fc Tag, low endotoxin

明星产品

1. FITC-Labeled Human CD19, Fc Tag蛋白(Cat. No. CD9-HF251
独有产品
> 适用于Anti-CD19-CAR阳性表达率检测
图1. 293 cells were transfected with anti-CD19-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251, 10 µg/ml) and C. FITC-labeled protein control. A. Non-transfected 293 cells and C. FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-CD19-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251)
点击申请Protocol
点击联系我们
> 标记前后活性一致
图2. Binding activity of the Human CD19, Fc Tag before and after FITC labeling were evaluated in the above ELISA analysis. The result showed that FITC-Labeled and Unconjugated Human CD19, Fc Tag have almost the same binding activity.
如需了解产品更多信息,可直接拨打:400-682-2521
2. FITC-Labeled Human CD19, His Tag蛋白(Cat. No. CD9-HF2H2
> 适用于Anti-CD19-CAR阳性表达率检测
图3. 293 cells were transfected with anti-CD19-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human CD19 (20-291) (Cat. No. CD9-HF2H2, 10 µg/ml) and C. FITC-labeled protein control. A. Non-transfected 293 cells and C. FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-CD19-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human CD19 (20-291) (Cat. No. CD9-HF2H2).
点击申请Protocol
点击联系我们
> 标记前后活性一致
图4. Binding activity of the Human CD19, His Tag before and after FITC labeling were evaluated in the above ELISA analysis. The result showed that the FITC-Labeled and Unconjugated Human CD19, His Tag have almost the same binding activity.
如需了解产品更多信息,可直接拨打:400-682-2521
> 高活性
图5. Binding activity of FITC-Labeled Human CD19, His Tag from two different vendors were evaluated in the above ELISA analysis against FMC63 MAb. The result showed that ACRO's FITC-Labeled Human CD19, His Tag has a much higher binding activity than Other Vendor's.
图6. Binding activity of FITC-Labeled Human CD19, His Tag from two different vendors were evaluated in the above flow cytometry analysis against anti-CD19-CAR-293 cells. The result showed that ACRO's FITC-Labeled Human CD19, His Tag has a much higher binding activity than Other Vendor's.
如需了解产品更多信息,可直接拨打:400-682-2521
3. 高亲和力Human CD19, Fc Tag蛋白(Cat. No. CD9-H5251
> 高活性
图7. Binding activity of Human CD19, Fc Tag from two different vendors were evaluated in the above ELISA analysis against FMC63 MAb. The result showed that ACRO's Human CD19, Fc Tag has a higher binding activity than Other Vendor's.
图8. Binding activity of Human CD19, Fc Tag from two different vendors were evaluated in the above flow cytometry analysis against anti-CD19-CAR-293 cells. The result showed that ACRO's Human CD19, Fc Tag has a higher binding activity than Other Vendor's.
如需了解产品更多信息,可直接拨打:400-682-2521
图9. Binding activity of Human CD19, Fc Tag from two different vendors were evaluated by SPR assay against FMC63 MAb. The result showed that ACRO's Human CD19, Fc Tag can bind FMC63 MAb with an affinity constant of 0.17 nM which is 2-fold higher than Other Vendor's Human CD19, Fc Tag.
如需了解产品更多信息,可直接拨打:400-682-2521
> 高纯度
图10. The purity of Human CD19, Fc Tag from two different vendors were determined in HPLC analysis. The result showed that the purity of ACRO’s Human CD19, Fc Tag is greater than 87%, while the purity of Other Vendor’s Human CD19, Fc Tag is greater than 57%.
如需了解产品更多信息,可直接拨打:400-682-2521
4. Human CD19, His Tag蛋白(Cat. No. CD9-H52H2
> 高活性
图11. Binding activity of Human CD19, His Tag from two different vendors were evaluated in the above ELISA analysis against FMC63 MAb. The result showed that ACRO's Human CD19, His Tag has a much higher binding activity than Other Vendor's.
图12. Binding activity of Human CD19, His Tag from two different vendors were evaluated in the above flow cytometry analysis against anti-CD19-CAR-293 cells. The result showed that ACRO's Human CD19, His Tag has a much higher binding activity than Other Vendor's.
如需了解产品更多信息,可直接拨打:400-682-2521
图13. Binding activity of Human CD19, His Tag from two different vendors were evaluated by SPR assay against FMC63 MAb. The result showed that ACRO's Human CD19, His Tag can bind FMC63 MAb with an affinity constant of 2.95 nM which is much higher than Other Vendor's Human CD19, His Tag.
如需了解产品更多信息,可直接拨打:400-682-2521

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