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抑制剂筛选试剂盒

背景
抑制剂筛选在药物开发中起着至关重要的作用。特别是在早期研发阶段,涉及到对大量候选分子进行筛选及优化,以确定具有显著药再对其深入评估。为了高效地筛选和验证多个潜在抑制剂,我们需要快速且灵敏度高的工具。
为了满足这一需求,ACROBiosystems百普赛斯基于竞争ELISA方法,建立了抑制剂筛选平台,利用内部独特的生物素蛋白标记技术,开发了一系列高通量、高灵敏度抑制剂筛选试剂盒,可用于前期抗体及小分子抑制剂筛选以及QC放行,节约建立方法所用的时间,帮助科研人员快速进行筛选和验证药物,有效提高研发效率。
概览
规格 96 Tests
检测方法 显色法
方法类型 竞争ELISA
应用 高通量筛选抗体、小分子抑制剂、多肽等。
产品特征

准确可靠:试剂盒原料经过验证,批间一致性高,保证实验结果稳定可靠,更有利于长期开发优化验证实验。

快速高效:通过使用生物素标记蛋白,减少标记过程,省时省力;方法充分验证,节省方法建立及验证时间。

降低干扰:基质干扰小,验证DMSO对体系造成的干扰,确保可以用来筛选小分子及大分子抑制剂。

高重复性:生物素标记蛋白采用定点标记,更稳定的标记方法保证了批间一致性及试剂盒的稳定性。

实验原理
应用竞争ELISA实验原理(不同抑制剂筛选试剂盒之间的原理略有不同)
图1. 抑制剂筛选试剂盒原理
用PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Kit(Cat. No. EP-101)作为例子进行分析:
实验分为四步:
  • 包被PD-L1于板孔中;
  • 加入生物素化的Human PD-1,结合板孔中的PD-L1;
  • 加入需要筛选的抑制剂;
  • 加入Streptavidin-HRP进行显色。
最后,通过比较不同实验组之间的OD读数,可以确定抑制剂抑制PD-1:PD-L1结合的能力
组分
以PD-1 [Biotinylated] : PD-L1抑制剂筛选试剂盒 (EP-101) 为例。
ID Components Size
EP101-C01 High-bind Plate 1 plate
EP101-C02 Human PD-L1 25 μg
EP101-C03 Anti-PD-1 Neutralizing Antibody 20 μg
EP101-C04 Human PD-1-Biotin 5 μg
EP101-C05 Streptavidin-HRP 10 μg
EP101-C06 Coating Buffer 12 mL
EP101-C07 10xWashing Buffer 50 mL
EP101-C08 Blocking Buffer 50 mL
EP101-C09 Substrate Solution 12 mL
EP101-C10 Stop Solution 7 mL
产品列表
货号 产品详情 检测方法 订购/预购
EP-101 PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Kit 显色反应

订购

EP-102 CD47: SIRP alpha [Biotinylated] Inhibitor Screening ELISA Assay Pair 显色反应

订购

EP-103 PCSK9 [Biotinylated] : LDL R Inhibitor Screening ELISA Assay Pair 显色反应

订购

EP-116 B7-1 [Biotinylated] : CTLA-4 Inhibitor Screening ELISA Kit 显色反应

订购

EP-117 B7-2 [Biotinylated] : CTLA-4 Inhibitor Screening ELISA Kit 显色反应

订购

EP-118 BCMA [Biotinylated] : BAFF Inhibitor Screening ELISA Kit 显色反应

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EP-119 BAFFR [Biotinylated] : BAFF Inhibitor Screening ELISA Kit 显色反应

订购

EP-120 PD-1 [Biotinylated] : PD-L2 Inhibitor Screening ELISA Kit 显色反应

订购

EP-121 CD27L [Biotinylated] : CD27 Inhibitor Screening ELISA Kit 显色反应

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EP-122 PVRIG : Nectin-2[Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-125 CTLA-4 [Biotinylated] : B7-1 Inhibitor Screening ELISA Kit 显色反应

订购

EP-126 CTLA-4 [Biotinylated] : B7-2 Inhibitor Screening ELISA Kit 显色反应

订购

EP-127 BCMA [Biotinylated] : APRIL Inhibitor Screening ELISA Kit 显色反应

订购

EP-128 IL-5[Biotinylated]:IL-5Rα Inhibitor Screening ELISA Kit 显色反应

订购

EP-129 TSLP[Biotinylated]:IL-7Rα & TSLP R Inhibitor Screening ELISA Kit 显色反应

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EP-130 PD-1 : PD-L1 [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-131 TIGIT : CD155 [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

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EP-132 IL-4 [Biotinylated] : IL-4Rα Inhibitor Screening ELISA Kit 显色反应

订购

EP-133 PD-L1 : PD-1 [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-134 PD-L1 [Biotinylated] : B7-1 Inhibitor Screening ELISA Kit 显色反应

订购

EP-135 CD30 [Biotinylated] : CD30L Inhibitor Screening ELISA Kit 显色反应

订购

EP-137 VEGFA : VEGFR2 [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-138 CD47 [Biotinylated] : SIRP alpha Inhibitor Screening ELISA Kit 显色反应

订购

EP-139 IL-17A [Biotinylated] : IL-17RA Inhibitor Screening ELISA Kit 显色反应

订购

EP-140 IL-17A : IL-17RA [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-141 VEGFA [Biotinylated] : VEGFR2 Inhibitor Screening ELISA Kit 显色反应

订购

EP-142 IL-4Rα [Biotinylated] : IL-4 Inhibitor Screening ELISA Kit 显色反应

订购

EP-143 TNF-alpha [Biotinylated] : TNFR1 Inhibitor Screening ELISA Kit 显色反应

订购

EP-144 TNFSF11 : RANK Inhibitor Screening ELISA Kit 显色反应

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EP-145 TNFSF11 : Osteoprotegerin Inhibitor Screening ELISA Kit 显色反应

订购

EP-146 IL-6 [Biotinylated] : IL-6 R alpha Inhibitor Screening ELISA Kit 显色反应

订购

EP-147 TNF-alpha [Biotinylated] : TNFR2 Inhibitor Screening ELISA Kit 显色反应

订购

EP-148 LIGHT : HVEM [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-149 IL-13RA2 [Biotinylated] : IL-13 Inhibitor Screening ELISA Kit 显色反应

订购

EP-150 CD28 : B7-1 [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-151 CD28 : B7-2 [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-152 CD47 [Biotinylated] : SIRP alphaV2 Inhibitor Screening ELISA Kit 显色反应

订购

EP-153 LIGHT : LTBR [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-154 PD-1 : PD-L2 [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-155 Integrin α 4 β 7 : MAdCAM-1 [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-156 IL-12B : IL-12RB1 [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-157 Mouse PD-L1 : Mouse PD-1 [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-158 PD-L1 [Biotinylated] : PD-1 Inhibitor Screening ELISA Kit 显色反应

订购

EP-159 IL-11 : IL-11R [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-160 CD47 : SIRP gamma [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-161 TNF-alpha : TNFR2 [Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

EP-162 OX40 [Biotinylated] : OX40 Ligand Inhibitor Screening ELISA Kit 显色反应

订购

EP-163 PD-1 : PD-L1 [Biotinylated] Inhibitor Screening ELISA Kit-Pre coated plate 显色反应

订购

EP-164 GDF15 [Biotinylated] : GFRAL Inhibitor Screening ELISA Kit 显色反应

订购

EP-165 TNF-alpha : TNFR1[HRP] Inhibitor Screening ELISA Kit-Pre coated plate 显色反应

订购

EP-166 CD73 Inhibitor Screening Kit 显色反应

订购

EP-167 IL-23 : IL-23R[Biotinylated] Inhibitor Screening ELISA Kit 显色反应

订购

验证数据

使用PD-1 [Biotinylated] : PD-L1 抑制剂筛选试剂盒(Cat. No. EP-101)来检测抗PD-1中和抗体对PD-1: PD-L1结合的抑制效果

Anti-PD-1 neutralizing antibody was diluted from 10 μg/mL to 0.078 μg/ mL (69.628 nM to 0.544 nM) and loaded onto the plate coated by human PD-L1 in the presence of human PD-1-Biotin. Assay was performed according to the protocol in PD-1[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

申请 Protocol

使用CD47: SIRP alpha [biotinylated]抑制剂筛选试剂盒(Cat. No. EP-102)来检测抗CD47中和性抗体对CD47: SIRP alpha结合的抑制效果

Serial dilutions of anti-CD47 neutralizing antibody (Catalog # CD7-NA002) (1:2 serial dilutions, from 10 μg/mL to 0.04 μg/mL or 66.67 nM to 0.26 nM) was added into CD47: biotinylated SIRP alpha binding reactions. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

申请 Protocol

使用IL-4 [biotinylated]: IL-4 R alpha抑制剂筛选试剂盒(Cat. No. EP-132) 来检测Human IL-4对IL-4: IL-4 R alpha结合的抑制效果

Serial dilutions of Human IL-4(Catalog # EP132-C03) (1:1 serial dilution, from 5 μg/mL to 0.01 μg/mL (295.86-0.58 nM)) was added into IL-4 R alpha: IL-4-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

申请 Protocol

使用Integrin α 4 β 7: MAdCAM-1 [Biotinylated]抑制剂筛选试剂盒(Cat. No. EP-155) 检测抗Integrin α 4 β 7中和抗体对Integrin α 4 β 7: MAdCAM-1结合的抑制效果

Serial dilutions of Anti-Integrin α 4 β 7 Neutralizing antibody (Catalog # EP155-C04) (1:1 serial dilution, from 5 μg/mL to 0.0098μg/mL) was added into Integrin α 4 β 7: Biotinylated MAdCAM-1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

申请 Protocol

使用CD73抑制剂筛选试剂盒(Cat. No. EP-166)检测CD73抑制剂对CD73: AMP结合的抑制效果

Serial dilutions of AMP-CP (1:1 serial dilution, from 500μm/L to 0.244140625μm/L) was added into CD73: AMP binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

申请 Protocol

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