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>产品&服务 > CD39/CD73:肿瘤免疫腺苷酸通路明星靶点

CD39/CD73:肿瘤免疫腺苷酸通路明星靶点

背景
肿瘤微环境(TME)是肿瘤免疫治疗的重要影响因素之一,ATP/ ADO(腺苷/adenosine)通路是肿瘤微环境调控的主要通路,而CD39/CD73在ATP/ ADO(腺苷/adenosine)通路中至关重要,在先天性和适应性免疫中发挥着重要作用。
其中CD39是一种跨膜三磷酸腺苷二磷酸水解酶-1(Ectonucleoside triphosphate diphosphohydrolase-1,ENTPD1),具有两个跨膜结构域和一个胞外结构域,其活性中心在胞外结构域上,两个跨膜结构域之间的互相作用也可能影响其酶活性。CD39在多种免疫细胞(NK细胞、CD4+ 、CD25+ 、调节性T细胞 (Treg)、巨噬细胞等),非免疫细胞( 内皮细胞和成纤维细胞)以及肿瘤细胞中( 淋巴瘤、肉瘤、肺癌等)中均有表达,由此奠定其在肿瘤免疫中的基础作用。
CD73是一种胞外-5′-核苷酸酶(NT5E),以同源二聚体形式存在于细胞表面,也可以游离形式存在于胞外。CD73主要由3个结构域组成,N端结构域,包含2个锌离子结合位点,C端结构域含底物结合位点, N端和C端结构域之间的短螺旋可控制酶的运动。其广泛表达于人体多种组织细胞表面,包括淋巴细胞、内皮细胞和上皮细胞,控制多种生理活动。
在其功能上,CD39能与胞外ATP(eATP)结合,并将其水解为AMP; CD73则会将AMP水解为ADO。在ATP-ADO通路中,CD39是eATP水解的限速酶,而CD73是AMP水解的限速酶。通过CD39CD73来控制嘌呤核苷酸的生成,进而调控核苷酸的信号传递。CD39可将能有效激活免疫细胞活性的eATP水解进而造成肿瘤的免疫逃逸;而CD73水解产生的ADO可活化免疫细胞,降低T细胞的免疫作用,进而导致肿瘤免疫逃逸。
因此,以CD39/CD73为靶点的大小分子药物的研发,成为调控肿瘤微环境ATP/ ADO(腺苷/adenosine)通路的新思路,进而为肿瘤免疫治疗提供新方向。 为加速CD39/CD73药物研发,ACROBiosystems提供CD39CD73系列产品,纯度和生物活性经过验证并且可以免费提供活性验证Protocol,可应用于免疫、抗体筛选等应用场景,加速CD39/CD73药物研发。
产品特点

多种属:Human, Mouse, Cynomolgus, Rabbit, Canine,可全面应用于不同种属交叉实验

高纯度:SDS-PAGE验证纯度>95%,SEC-MALS验证纯度>90%

多样化产品选择:预偶联磁珠

高酶活:提高底物催化效率,从而有利于药物筛选

产品高生物活性经ELISA/SPR/BLI验证

高批间一致性:严格质控为高批间一致性保驾护航

除了重组蛋白相关以外,ACRO 基于其独特的生物素标记蛋白产品,将生物素化蛋白偶联到链霉亲和素磁珠上,设计开发了独特的预偶联磁珠产品,可用于免疫捕获、生物淘选、样品/细胞富集
产品列表
分子名 货号 物种 产品描述
CD73CD3-H5252HumanHuman CD73 Protein, Mouse IgG2a Fc Tag (active enzyme)
CD3-H52H7HumanHuman CD73 / NT5E Protein, His Tag (HPLC-verified) (active enzyme)HOT
CD3-H82E3HumanBiotinylated Human CD73 / NT5E Protein, His,
CD3-M52H9MouseMouse CD73 / NT5E Protein, His Tag (active enzyme)HOT
CD3-C52H9CynomolgusCynomolgus CD73 Protein, His Tag (active enzyme)
CD3-R52H5RatRat CD73 Protein, His Tag (active enzyme)
CD3-R52H3RabbitRabbit CD73 / NT5E Protein, His Tag (active enzyme)NEW
CD3-C52H5CanineCanine CD73 / NT5E Protein, His Tag (MALS verified) (active enzyme)
CD3-S52H3Sus scrofa (Pig)Sus scrofa CD73 / NT5E Protein, His Tag (active enzyme, MALS verified)
CEA-B048HumanClinMax™ Human Soluble CD73 / NT5E ELISA Kit, PRO
EP-166HumanCD73 Inhibitor Screening Kit
MBS-K022HumanHuman CD73-coupled Magnetic Beads
CD39CD9-H5253HumanHuman CD39 Protein, Mouse IgG2a Fc Tag (active enzyme)
CD9-H52H4HumanHuman CD39 / ENTPD1 Protein, His Tag (active enzyme, MALS verified)HOT
CD9-H52H5HumanHuman CD39 / ENTPD1 (Q96A, N99A, E143A, R147E) Protein, His Tag (active enzyme, MALS verified)
CD9-H52H7HumanHuman CD39 (R138A, M139A, E142K) Protein, His Tag (active enzyme)
CD9-H82E5HumanBiotinylated Human CD39 / ENTPD1 Protein, His, (active enzyme)
CD9-H82E8HumanBiotinylated Human CD39 (R138A, M139A, E142K) Protein, His, (active enzyme)
CD9-M52H3MouseMouse CD39 / ENTPD2 Protein, His Tag (active enzyme, MALS verified)
CD9-C5PH3CynomolgusCynomolgus CD39 Protein, His Tag (active enzyme, MALS verified)NEW
*如果您希望ACRO开发更多类型的腺苷酸通路蛋白产品, 请 点击提议
*如果您希望ACRO补充更多CD39/CD73蛋白产品的实验设计和应用数据,请 点击咨询
产品验证数据
高纯度经MALS验证

The purity of Canine CD73, His Tag (Cat. No. CD3-C52H5) is more than 90% and the molecular weight of this protein is around 115-141 kDa verified by SEC-MALS.

Report

高生物活性
ELISA

Immobilized Human CD39, His Tag (Cat. No. CD9-H52H4) at 1 μg/mL (100 μL/well) can bind Anti-CD39 Antibody, Human IgG1 with a linear range of 0.1-0.8 ng/mL (QC tested).

Protocol

Immobilized Monoclonal Anti-Human CD73 Antibody, Human IgG1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD73, His,Avitag (Cat. No. CD3-H82E3) with a linear range of 0.6-5 ng/mL (QC tested).

Protocol

BLI

Loaded Anti-CD73 MAb (Human IgG1) on AHC Biosensor, can bind Human CD73, His Tag (HPLC-verified) (Cat. No. CD3-H52H7) with an affinity constant of 0.179 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

SPR

Anti-CD73 antibody (Human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CD73, His Tag (HPLC-verified) (Cat. No. CD3-H52H7) with an affinity constant of 0.164 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

高酶活
测定产品 Human CD39 / ENTPD1 Protein, His Tag Human CD73 / NT5E Protein, His Tag (HPLC-verified)
水解底物 腺苷-5 ' -三磷酸(ATP) 腺苷-5 ' -单磷酸(AMP)
酶活
(pmol/min/μg)
ACRO产品
(货号:CD9-H52H4
竞品  ACRO产品
(货号:CD3-H52H7
竞品 
> 6,000 >5,000 >100,000  >15,000
测定产品 Mouse CD39 / ENTPD2 Protein, His Tag (MALS verified) Mouse CD73 / NT5E Protein, His Tag (active enzyme)
水解底物 腺苷-5 ' -三磷酸(ATP) 腺苷-5 ' -单磷酸(AMP)
酶活
(pmol/min/μg)
ACRO产品
(货号:CD9-M52H3
竞品  ACRO产品
(货号:CD3-M52H9
竞品 
> 25,000 >25,000 >20,000  >20,000 
测定产品 Cynomolgus CD39 / ENTPD1 Protein, His Tag (MALS verified) (active enzyme) Cynomolgus CD73 Protein, His Tag (active enzyme)
水解底物 腺苷-5 ' -三磷酸(ATP) 腺苷-5 ' -单磷酸(AMP)
酶活
(pmol/min/μg)
ACRO产品
(货号:CD9-C5PH3
竞品  ACRO产品
(货号:CD3-C52H9
竞品 
>15,000 >5,000 >50,000   >20,000

注:同一产品在相同条件下测定酶活

预偶联生物素化CD73蛋白磁珠(货号:MBS-K022

Immobilized 62 μg CD73 protein to 1 mg Beads can bind the Anti-CD73 Antibody with an EC50 of 0.5659 μg/mL (QC tested).

Protocol


热门评论
ACROBiosystems提供的高质量CD39/CD73系列蛋白产品客户反馈良好。
 
评论(4)
  1. 136XXXXXXX6
  2. 18人赞
  3. 购买蛋白用于测试CD39封闭性抗体封闭CD39水解ATP的功能实验。通过系列梯度CD39抗体与一定浓度的CD39(0.5ug/ml)孵育,再加入50uM ATP,用CTG检测,得到很好的曲线。证明该蛋白具有很好的ATP酶水解功能。
  4. 2019-11-11
  1. 182XXXXXXX2
  2. 8人赞
  3. Human CD73用来检测medimmune家的抗体与其亲和力,结果具有很高的亲和力,亲和力为5E-10.
  4. >
  5. 2020-01-10
  1. 182XXXXXXX2
  2. 4人赞
  3. Mouse CD73到货后就立即开始使用啦,虽然是鼠的,但是与抗体的结合力依然很强,BLI数据显示亲和力有6E-10.
  4. >
  5. 2020-01-10
  1. 82-XXXXXXX-8440
  2. 2人赞
  3. It was used in research of investgational new drugs. Specifically, I have used this product in my Epitope binning experiments (using Octet) and I am truely satisfied with the results. With this experiment and your product, we have found a approximate epitope binding site. Thanks for your highly qualified product.
  4. 2021-12-18
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