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CD24 & Siglec-10蛋白 – 肿瘤免疫治疗热门靶点

背景
癌细胞表面表达特定的“别吃我”的信号分子(如CD24、CD47、PD-L1、B2M)后会被免疫细胞识别为正常细胞,从而逃避肿瘤微环境中巨噬细胞的吞噬清除。最早发现的“别吃我”信号蛋白是PD-1和CTLA-4,2019年7月发表在Nature的一项研究表明CD24是卵巢癌和三阴乳腺癌细胞表达的另一个“别吃我”信号蛋白,被癌细胞用来保护自己,CD24是开发癌症免疫疗法潜在靶点。
CD24(Cluster of Differentiation 24)是一种高度糖基化的糖基磷脂酰肌醇锚定的细胞表面蛋白。卵巢癌和三阴乳腺癌细胞表面上表达的CD24蛋白可以与免疫细胞上的Siglec-10蛋白相互作用来传递免疫抑制性信号,从而抑制破坏性炎症反应。
目前,市面的CD24蛋白大多都只有与抗体结合的数据,缺少与Siglec-10蛋白结合活性的验证,而CD24抗体药研发的关键点在于其能够有效地阻断CD24与Siglec-10的结合。为了解决这些问题,ACROBiosystems开发了不同标签的CD24蛋白以及CD24过表达细胞株,并验证了细胞株与Siglec-10的结合活性。另外还验证了抗CD24中和抗体SN3可以有效的阻断Siglec-10与CD24过表达细胞株的结合。
产品列表
Molecule Cat. No. Species Product Description
CD24 CD4-H52H3 Human Human CD24 Protein, His Tag
CD4-H5254 Human Human CD24 Protein, Fc Tag
CD4-H5257 Human Human CD24 Protein, Mouse IgG2a Fc Tag
CD4-H82E9 Human Biotinylated Human CD24 Protein, His,Avitag™
CD4-H82F5 Human Biotinylated Human CD24 Protein, Fc,Avitag™
CD4-M52H7 Mouse Mouse CD24 Protein, His Tag
Siglec-10 SI0-H52H9 Human Human Siglec-10 Protein, His Tag
SI0-H5253 Human Human Siglec-10 Protein, Fc Tag
SI0-H525b Human Human Siglec-10 Protein, Mouse IgG2a Fc Tag, low endotoxin
SI0-H82E3 Human Biotinylated Human Siglec-10 Protein, His,Avitag™
SI0-H82F6 Human Biotinylated Human Siglec-10 Protein, Fc,Avitag™
SI0-M52H7 Mouse Mouse Siglec-10 Protein, His Tag
实验数据
CD24过表达细胞株与Siglec-10的结合验证
PE Labeled Siglec-10 Protein
Fig.1 FACS assay showed that Human Siglec-10 can bind to CD24 overexpressed cells.

Protocol

抗CD24抗体(SN3)抑制Siglec-10与CD24过表达细胞株的结合
Inhibition of Anti-CD24 Antibody (SN3)
Fig.2 FACS assay showed that the binding signal of Siglec-10 and CD24 overexpressed cells was inhibited by anti-CD24 neutralizing antibody.

Protocol

相关研究进展

CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy.

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