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 >  Protein>IL-17A >CT8-M5240

Mouse IL-17A / CTLA-8 Protein, His Tag (MALS verified)

分子别名(Synonym)

IL-17A,Interleukin-17A,CTLA-8,IL-17

表达区间及表达系统(Source)

Mouse IL-17A, His Tag (CT8-M5240) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Ala 158 (Accession # Q62386-1).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

IL-17A Structure

This protein carries a polyhistidine tag at the N-terminus

The protein has a calculated MW of 15.8 kDa. The protein migrates as 17-19 kDa and 21-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-17A SDS-PAGE

Mouse IL-17A, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

IL-17A SEC-MALS

The purity of Mouse IL-17A, His Tag (Cat. No. CT8-M5240) is more than 85% and the molecular weight of this protein is around 35-50 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

IL-17A ELISA

Immobilized Mouse IL-17A, His Tag (Cat. No. CT8-M5240) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) with a linear range of 4-125 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-BLI

IL-17A BLI

Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Mouse IL-17A, His Tag (Cat. No. CT8-M5240) with an affinity constant of 20.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性(Bioactivity)-Bioactivity CELL BASE

IL-17A CELL

Mouse IL-17A, His Tag (Cat. No. CT8-M5240) stimulates secretion of IL-6 by NIH-3T3 cells. The ED50 for this effect is 2.132-5.005 ng/mL.

Protocol

 

背景(Background)

Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

 

前沿进展

 
 
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IL-17A靶点信息
英文全称:Interleukin 17A
中文全称:白细胞介素-17A
种类:Homo sapiens
上市药物数量:3详情
临床药物数量:27详情
最高研发阶段:批准上市
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