BPM CD Feed CHO MAX-1 is specifically designed for recombinant CHO cells (CHO-K1, DG44, DXB11, CHO-S, and GS CHO) as supplement in fed-batch culture. It is high effective to make grams level volumetric productivity of your cell culture process. CD Feed CHO MAX-1 is suitable for cell culture process of pilot and commercial scale production of antibody/protein. CD Feed CHO MAX-1 is an animal origin-free, chemically defined medium that contains no proteins, polypeptides, hydrolysates, or components of unknown composition.
- Designed to work with recombinant CHO cell line as fed batch supplement
- 3 - 6 fold increases versus batch culture process
- Boost productivity of selected CHO cell lines
- Protein free, animal component free, chemically define and regulatory friendly
- General Specification
- Cell Culture Conditions
|Concentrated||Cell Line||Form||Serum||Endotoxin||Glutamine||Culture Type||Phenol Red|
|10X||CHO||Liquid||Serum Free||Low||No||Suspension Culture||No|
|Product Classification||Buffer system||Regulatory Statement||Shelf Life|
|Protein-Free, Chemically Defined;Serum-Free, Animal Origin-Free||N/A||For Research and Further Cell Culture Manufacturing Use Only||12months|
|Cat. No.||Name of Product||Product Size||Storage||Shipping|
|CF-1120-11||CD Feed CHO MAX-1||100mL||at +2-8℃, in dark||Ice pack|
|CF-1120-12||CD Feed CHO MAX-1||500mL||at +2-8℃, in dark||Ice pack|
|CF-1120-13||CD Feed CHO MAX-1||1000mL||at +2-8℃, in dark||Ice pack|
- CD Feed CHO MAX-1 requires supplementation of L-glutamine according to your cell line's nutrient needs.
- Feeding strategy should be determined for specific process, 8 - 15%(V/V) total addition is recommended for initially test.
- Adjust pH to 7.0 using 1N NaOH after reconstitution prior to use.
细胞培养环境（Cell Culture Conditions）
Standard cell culture conditions of feeding CHO cell culture with CD Feed CHO MAX-1 are 36 to 38°C in a humidified air of 5-8% CO2. Under aseptic and cultivation conditions, cell culture vessel can be shaker flask, spinner flask and Wave or any other stirred bioreactors. Shaker flask volume can be from 125 mL to 1000 mL with approximate 20% to 30% working volume in suspension, and an orbital shaker platform rotating at 115 to 150 rpm is recommended. Loosen caps of flasks or use with vented caps for gas exchange is required for high density cell growth. Avoid overexposure of cultures to light.
|Cat. No.||Product Name||Descriptions|
|CM-1121||CDPro CHO-1 Medium||Specially designed for transfected CHO cells|
|CM-1122||CDPro CHO-2 Medium||Specially designed for CHO-K1,CHO-S and GS CHO|
|CM-1123||CDPro CHO-3 Medium||Universal CHO medium|
|CM-1124||PFPro CHO-1 Medium||Specially designed for DHFR deficient CHO cells|
|CM-1125||PFPro CHO-2 Medium||Easy adaptation CHO medium(coming soon)|
|CF-1121||CD Feed CHO MAX-2||Concentrated supplement for fed batch process|