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CDPro 293-1 Medium, 1X, Liquid


产品描述(Descripiton)

BPM CDPro 293-1 Medium is specifically designed and optimized for recombinant 293 cells (293F, 293 E1, 293T, 293A, 293FT, HEK293) for high density of suspension culture. CDPro 293-1 medium is suitable for cell culture process of pilot and commercial scale production of protein and adenovirus, influenza virus or viral vectors. CDPro 293-1 is an animal origin-free, chemically defined medium that contains no proteins, polypeptides, hydrolysates, or components of unknown composition. This medium also supports PER.C6® cell growth, infection of virus and further production.

产品特性(Product Features)

  1. Designed for suspension  culture of 293 cells
  2. For application of protein expression and virus production in 293 cells
  3. Low osmolality characteristics facilitate fed batch process development and optimization
  4. Protein free, animal component free, chemically define and regulatory friendly
  5. Extensive support by cell culture expert
  • General Specification
  • Precaution
  • Cell Culture Conditions

产品参数(General Specification)

Concentrated Cell Line Form Serum Endotoxin Glutamine Culture Type Phenol Red
1X 293 Liquid Serum Free Low No Suspension Culture Yes
Product Classification Buffer system Regulatory Statement Shelf Life
Protein-Free, Chemically Defined;Serum-Free,   Animal Origin-Free Sodium Bicarbonate  For Research and Further Cell Culture Manufacturing Use Only 18 months
Cat. No. Name of Product Product Size Storage Shipping
CM-1131-11 CDPro 293-1 Medium 500mL at +2-8℃, in dark Ice pack
CM-1131-12 CDPro 293-1 Medium 1000mL at +2-8℃, in dark Ice pack
CM-1131-13 CDPro 293-1 Medium 10L at +2-8℃, in dark Ice pack

注意事项(Precaution)

  1. CDPro 293-1 requires supplementation of L-glutamine or Ala-Gln dipeptide prior to use.
  2. L-glutamine level must be considered in fed batch or perfusion model or your cells are sensitive to ammonia which is more likely generated in presence of  high concentration of glutamine.

细胞培养环境(Cell Culture Conditions)

Standard cell culture conditions of growing 293 cells in CDPro 293-1 medium are 36 to 38°C in a humidified air of 5-8% CO2. Under aseptic and cultivation conditions, cell culture vessel can be shaker flask, spinner flask and Wave or any other stirred bioreactors. Shaker flask volume can be from 125 mL to 1000 mL with approximate 20% to 30% working volume in suspension, and an orbital shaker platform rotating at 115 to 150 rpm is recommended. Loosen caps of flasks or use with vented caps for gas exchange is required for high density cell growth. Avoid overexposure of cultures to light.

相关产品(Related Products)

Cat. No. Product Name Descriptions
CM-1121 CDPro CHO-1 Medium Specially designed for transfected CHO cells
CM-1122 CDPro CHO-2 Medium Specially designed for CHO-K1,CHO-S and GS CHO
CM-1141 CDPro HM-1 Medium Specially designed for NS0 and Hybrimoma cell culture
CM-1156 CD 293 TGE Medium Serum free medium for transient transfection
CF-1120 CD Feed CHO MAX-1 Concentrated supplement for fed batch process
CF-1121 CD Feed CHO MAX-2 Concentrated supplement for fed batch process

前沿进展

货号/价格

CM-1131-13-10L / ¥5000

产品定制
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com

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