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CD 293 TGE Medium, Powder

产品描述(Descripiton)

CD 293 TGE Medium is a proprietary, chemically defined, animal origin-free medium specifically developed for the high-density, suspension culture and transfection of 293 cells. Transient expression of plasmid DNA in HEK293 cells adapted in this medium can get high volumetric productivity of target proteins. Used with BPM SBPfectin, 293 Expression MAXTM-1 and Feed X together is recommended for higher yield of protein expression.

CD 293 TGE Medium is specifically formulated for use with:

  1. Suspension HEK293(293T, 293EBNA, 293F) transient expression
  2. Suspension culture of HEK 293 stable cell cline for protein expression
  3. Large-scale, high-density growth of HEK293 cells in bioreactors

产品特性(Product Features)

  1. Support suspension HEK 293(293T, 293EBNA, 293F) transient expression
  2. >200% cell growth and expression performance compared competitive alternatives
  3. Prepared ready-to-use, with no supplementation required
  4. Protein free, Animal origin free, chemically defined and regulatory friendly
  5. Extensive support by cell culture expert
  • General Specification
  • Components
  • Usage Protocol

产品参数(General Specification)

Concentrated Cell Line Form Serum Endotoxin Glutamine Culture Type Phenol Red
1X 293 Powder Serum Free Low No Suspension Culture No
Product Classification Buffer system Regulatory Statement Shelf Life
Protein-Free, Chemically Defined;Serum-Free,   Animal Origin-Free Sodium Bicarbonate For Research and Further Cell Culture Manufacturing Use Only 24 months
Cat. No. Name of Product Product Size Storage Shipping
CM-1256-12 CD 293 TGE Medium, Powder 50L at +2-8℃, dry RT
Components ID Components Name Status Amount Usage Concentration Storage
CM-1256-A CD 293 TGE Medium Stock A Powder 747g x 1 14.94g per 1L 4-8 ℃
CM-1256-B CD 293 TGE Medium Stock B Liquid 1L x 3 60mL per 1L 4-8 ℃, avoid light

Note: Total amount of Stock A and B can produce 50L CD 293 TGE Medium

操作流程(Usage Protocol)

To prepare 1L CD 293 TGE Medium working solution as an example.

  1. Glutamine stock solution preparation: Weight 0.584g and add into 25mL deionized distilled water, with gentle stirring at room temperature until the powder has been fully dissolved. Sterilize immediately by membrane filtration. Store at -20℃ and avoid light. It is recommended to prepare the solution with the current use.
  2. Weight 14.94 g CD 293 TGE MediumStock A and add into 850mL deionized distilled water with gentle stirring at 20℃~42℃ until the powder has been fully dissolved.
  3. Pipette 60mL CD 293 TGE Medium Stock B under sterile conditions, and then add to dissolved CD 293 TGE Medium Stock A solution. Mix well
  4. Thaw the Glutamine stock solution in water bath at 37℃ before use. Pipette 25mL Glutamine stock solution under sterile conditions, and then add to the solution prerared in step 3.
  5. Mix well and dilute to a volume of 1L with deionized distilled water. Adjust pH of the solution to 7.0±0.1 by using 10M NaOH or 10M HCl . Mix well.
  6. Sterilize immediately by membrane filtration. Store prepared CD 293 TGE Medium working solution at 4℃ and avoid light.

相关产品(Related Products)

Cat. No. Product Name Quantity Storage Conditions
CM-1156-11 CD 293 TGE Medium, 1X, Liquid 1000 mL 2-8℃, in the dark
CM-1156-12 CD 293 TGE Medium, 1X, Liquid 6 X 1000 mL 2-8℃, in the dark
CM-1256-12 CD 293 TGE Medium, 1X, Powder 50 L 2-8℃, dry
CM-1157-11 CD CHO TGE Medium, 1X, Liquid 1000 mL 2-8℃, in the dark
CM-1157-12 CD CHO TGE Medium, 1X, Liquid 6 X 1000 mL 2-8℃, in the dark
TF-1157-11 BPfectin 1.5 mL -20℃
TF-1157-12 BPfectin 15 mL -20℃
EXP-711-11 293 Expression MAX-1 1 mL -20℃
EXP-811-11 CHO Expression MAX-1 1 mL -20℃
CF-1116-12 Feed X Supplement 500 mL 2-8℃, in the dark
AC-1112-11 Anti-Clumping Additive 1 mL 2-8℃, in the dark

前沿进展

货号/价格

CM-1256-12-50L / ¥15000

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