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Biotinylated Human TNFSF11 / RANKL / CD254 Protein, Fc Tag, Avi Tag (Avitag™)

分子别名(Synonym)

RANKL,CD254,TRANCE,OPGL,ODF

表达区间及表达系统(Source)

Biotinylated Human TNFSF11, Fc Tag, Avi Tag (RAL-H82F9) is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1).

Predicted N-terminus: Gly

蛋白结构(Molecular Characterization)

Online(Gly 64 - Asp 245) AAC51762.1

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.

The protein has a calculated MW of 49.2 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human TNFSF11, Fc Tag, Avi Tag (Cat. No. RAL-H82F9) SDS-PAGE gel

Biotinylated Human TNFSF11, Fc Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human TNFSF11, Fc Tag, Avi Tag (Cat. No. RAL-H82F9) with a linear range of 0.4-13 ng/mL (QC tested).

点击申请Protocol

Biotinylated Human SPR

Immobilized Human OPG, Fc Tag (Cat. No. TNB-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Human TNFSF11, Fc Tag, Avi Tag (Cat. No. RAL-H82F9) with a linear range of 0.3-10 ng/mL (Routinely tested).

点击申请Protocol

背景(Background)

Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is known as a type II membrane protein and is a member of the tumor necrosis factor (TNF) superfamily. RANKL, through its ability to stimulate osteoclast formation and activity, is a critical mediator of bone resorption and overall bone density. Some findings also suggestion some cancer cells, particularly prostate cancer cells, can activate an increase in bone remodeling and ultimately increase overall bone production.[17] This increase in bone remodeling and bone production increases the overall growth of bone metastasizes. The overall control of bone remodeling is regulated by the binding of RANKL with its receptor or its decoy receptor, respectively, RANK and OPG.

 

专利使用说明(Limited Use License)

The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

 

前沿进展

货号/价格

RAL-H82F9-25ug / ¥3500

RAL-H82F9-200ug / ¥13800

文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-31604788(上海)
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
TNFSF11靶点信息
英文全称:Tumor necrosis factor ligand superfamily member 11
中文全称:肿瘤坏死因子配体超家族成员11
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:14详情
最高研发阶段:批准上市
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