膜杰作
Star Staining
分子别名（Synonym）
CD112,Nectin-2,PVRL2
表达区间及表达系统（Source）
Human Nectin-2, Mouse IgG2a Fc Tag (CD2-H5257) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Leu 360 (Accession # Q92692-2).
Predicted N-terminus: Gln 32
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a mouse IgG2a Fc tag at the C-terminus
The protein has a calculated MW of 62.4 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Nectin-2, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) at 5 μg/mL (100 μL/well) can bind Human Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) with a linear range of 2-78 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human PVRIG, His,Avitag (Cat. No. PVG-H82E3) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, bind Human Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) with a linear range of 0.1-5 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) on Protein A Biosensor, can bind Human PVRIG, His Tag (Cat. No. PVG-H52H4) with an affinity constant of 1.33 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
Poliovirus receptor-related 2 (PVRL2) is also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), which is a human plasma membrane glycoprotein. PVRL2 is one of the plasma membrane components of adherens junctions. PVRL2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in PVRL2 gene have been associated with differences in the severity of multiple sclerosis. Also, PVRL2 binds with low affinity to TIGIT. Furthermore, PVRL2 acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).
