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Hamster PCSK9 Protein, His Tag

分子别名(Synonym)

PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9

表达区间及表达系统(Source)

Hamster PCSK9, His Tag (PC9-H52E4) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Ser 691 (Accession # G3GTK5).

Predicted N-terminus: Gln 30

蛋白结构(Molecular Characterization)

Online(Gln 30 - Ser 691) G3GTK5

This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.9 kDa and 59.0 kDa respectively. The protein migrates as 18 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Hamster PCSK9, His Tag (Cat. No. PC9-H52E4) SDS-PAGE gel

Hamster PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human LDL R, Strep Tag (Cat. No. LDR-H5281) at 5 μg/mL (100 μL/well) can bind Hamster PCSK9, His Tag (Cat. No. PC9-H52E4) with a linear range of 1-16 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human LDL R, Fc Tag (Cat. No. LDR-H5254) on Protein A Biosensor, can bind Hamster PCSK9, His Tag (Cat. No. PC9-H52E4) with an affinity constant of 4.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

 

前沿进展

 
货号/价格

PC9-H52E4-50ug / ¥2600

PC9-H52E4-1mg (500ug X 2) / ¥19800

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PCSK9靶点信息
英文全称:Proprotein convertase subtilisin kexin type 9
中文全称:前蛋白转化酶枯草杆菌蛋白酶Kexin-9
种类:Homo sapiens
上市药物数量:2详情
临床药物数量:28详情
最高研发阶段:批准上市
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