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Biotinylated Human CEACAM-6 / CD66c Protein, His,Avitag™ (MALS verified)

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分子别名(Synonym)

CEACAM6,CD66c,CEAL,NCA

表达区间及表达系统(Source)

Biotinylated Human CEACAM-6, His,Avitag (CE6-H82E7) is expressed from human 293 cells (HEK293). It contains AA Lys 35 - Gly 320 (Accession # P40199-1).

Predicted N-terminus: Lys 35

Request for sequence

蛋白结构(Molecular Characterization)

Online(Lys 35 - Gly 320) P40199-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 34.9 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human CEACAM-6, His,Avitag (Cat. No. CE6-H82E7) SDS-PAGE gel

Biotinylated Human CEACAM-6, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Biotinylated Human CEACAM-6, His,Avitag (Cat. No. CE6-H82E7) MALS images

The purity of Biotinylated Human CEACAM-6, His,Avitag (Cat. No. CE6-H82E7) is more than 90% and the molecular weight of this protein is around 48-66 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CEACAM-8, His Tag (Cat. No. CE8-H5224) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-6, His,Avitag (Cat. No. CE6-H82E7) with a linear range of 0.078-1.25 μg/mL (QC tested).

Protocol

背景(Background)

Carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen) (CEACAM6) is also known as CD66c (Cluster of Differentiation 66c), CEAL, NCA, and is one of seven human CEACAM family members within the immunoglobulin superfamily. In humans, CEACAMs include type I transmembrane proteins (CEACAM1, CEACAM3, and CEACAM4) and GPI-linked molecules (CEACAM5 through CEACAM8). There is no human CEACAM2. CEACAM 6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains. It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-selectin, galectins and some bacterial fimbrae. CEACAM-6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.

 

 

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CEACAM-6靶点信息
英文全称:Carcinoembryonic antigen related cell adhesion molecule 6
中文全称:癌胚抗原相关细胞粘附分子6
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床二期
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