登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>Mesothelin >MSN-HG2P4

GFP Fusion Human Mesothelin / MSLN (296-580) Protein, His Tag

分子别名(Synonym)

MSLN,Mesothelin,MPF

表达区间及表达系统(Source)

GFP Fusion Human Mesothelin (296-580), His Tag (MSN-HG2P4) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1 ).

Predicted N-terminus: Ala 2

Request for sequence

蛋白结构(Molecular Characterization)

Mesothelin Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 60.4 kDa. The protein migrates as 70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

偶联(Conjugate)

GFP

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 530 nm

应用说明(Application)

Flow cytometry (E.g. Evaluation of anti-MSLN CAR expression), fluorescence microscopy, etc.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mesothelin SDS-PAGE

GFP Fusion Human Mesothelin (296-580), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Mesothelin ELISA

Immobilized Anti-Human MSLN MAb at 10 μg/mL (100 μL/well) can bind GFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HG2P4) with a linear range of 0.039-0.625 μg/mL (QC tested).

Protocol

 

活性(Bioactivity)-SPR

Mesothelin SPR

Anti-human MSLN MAb (chimeric mouse-human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface, can bind GFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HG2P4) with an affinity constant of 0.542 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-FACS

Mesothelin FACS

2e5 of the MSLN-CAR-293 cells were stained with 100 μL of 3 μg/mL of GFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HG2P4). GFP was used as negative control (Routinely tested).

Protocol

 

背景(Background)

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
Mesothelin靶点信息
英文全称:Mesothelin
中文全称:间皮素
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:33详情
最高研发阶段:临床二期
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定