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Human CD73 / NT5E Protein, His Tag

分子别名(Synonym)

CD73,NT5E,5'-Nucleotidase,5'-NT,NT5,NTE

表达区间及表达系统(Source)

Human CD73, His Tag (CD3-H52H7) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # P21589-1).

Predicted N-terminus: Trp 27

蛋白结构(Molecular Characterization)

Online(Trp 27 - Ser 549) P21589-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 59.9 kDa. The protein migrates as 30 kDa, 40 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>85% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl, 4 mM CaCl2, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD73, His Tag (Cat. No. CD3-H52H7) SDS-PAGE gel

Human CD73, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

 

活性(Bioactivity)

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The specific activity is > 15,000 pmol/min/μg.

点击申请Protocol

 

背景(Background)

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

 

前沿进展

货号/价格

CD3-H52H7-20ug / ¥2500

CD3-H52H7-100ug / ¥7500

CD3-H52H7-500ug / ¥12000

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CD73靶点信息
英文全称:5'-nucleotidase
中文全称:5'-核苷酸酶
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床一期
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