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RFP Fusion Human Mesothelin / MSLN (296-580) Protein, His Tag

分子别名(Synonym)

MSLN,Mesothelin,MPF

表达区间及表达系统(Source)

RFP Fusion Human Mesothelin (296-580), His Tag (MSN-HR2P4) is expressed from human 293 cells (HEK293). It contains AA Glu 296-Gly 580 (Accession # AAH09272.1).

Predicted N-terminus: Glu 296

蛋白结构(Molecular Characterization)

Online(Glu 296-Gly 580)  AAH09272.1

This protein carries a RFP tag at the C-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 62.0 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

应用说明(Application)

Flow cytometry (E.g. Evaluation of anti-MSLN CAR expression), fluorescence microscopy, etc.


Conjugate

RFP

Excitation source: 561 nm spectral line, Yellow Green Laser

Excitation Wavelength: 561 nm

Emission Wavelength: 586/15 nm

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

RFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HR2P4) SDS-PAGE gel

RFP Fusion Human Mesothelin (296-580), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Anti-Human MSLN Mab, Human IgG1 at 10 μg/mL (100 μL/well) can bind RFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HR2P4) with a linear range of 0.078-0.625 μg/mL (QC tested).

点击申请Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Anti-human MSLN MAb (chimeric mouse-human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface, can bind RFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HR2P4) with an affinity constant of 0.304 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

点击申请Protocol

 

活性(Bioactivity)-FACS

Biotinylated Human FACS

2e5 of the MSLN-CAR-293 cells were stained with 100 μL of 3 μg/mL of RFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HR2P4). Non-transfected 293 cells were used as negative control (QC tested).

点击申请Protocol

 

背景(Background)

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

 

前沿进展

货号/价格

MSN-HR2P4-25ug / ¥3500

MSN-HR2P4-200ug / ¥13800

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Mesothelin靶点信息
英文全称:Mesothelin
中文全称:间皮素
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:8详情
最高研发阶段:临床二期
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