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 >  Protein>IL-15 R alpha >ILA-H82F4

Biotinylated Human IL-15 R alpha / CD215 Protein, Fc,Avitag™ (MALS verified)

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分子别名(Synonym)

IL-15 R alpha,CD215,IL15RA,IL-15RA,IL-15R-alpha

表达区间及表达系统(Source)

Biotinylated Human IL-15 R alpha, Fc,Avitag (ILA-H82F4) is expressed from human 293 cells (HEK293). It contains AA Ile 31 - Thr 205 (Accession # Q13261-1 ).

Predicted N-terminus: Ile 31

Request for sequence

蛋白结构(Molecular Characterization)

IL-15 R alpha Structure

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 46.6 kDa. The protein migrates as 60-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-15 R alpha SDS-PAGE

Biotinylated Human IL-15 R alpha, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

IL-15 R alpha SEC-MALS

The purity of Biotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ILA-H82F4) is more than 90% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

IL-15 R alpha ELISA

Immobilized Human IL-15, premium grade (Cat. No. IL5-H4117) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ILA-H82F4) with a linear range of 2-39 ng/mL (QC tested).

Protocol

IL-15 R alpha ELISA

Immobilized Mouse IL-15, Fc Tag (Cat. No. IL5-M5255) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ILA-H82F4) with a linear range of 10-156 ng/mL (Routinely tested).

Protocol

 

背景(Background)

IL15R is a heterotrimer of IL15RA, IL2RB and IL2RG. IL-15RA, also known as CD215, IL-15 R alpha, is a widely expressed 60 kDa transmembrane glycoprotein. High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Expression of different isoforms may alter or interfere with signal transduction. An interleukin (IL)-15 superagonist/IL-15 receptor α fusion complex (IL-15SA/IL-15RA; ALT-803) activates the IL-15 receptor on CD8 T cells and NK cells, and has shown significant anti-tumor. Signal transduction involves SYK. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.

 

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IL-15 R alpha靶点信息
英文全称:Interleukin-15 receptor subunit alpha
中文全称:白细胞介素-15受体α亚基
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:17详情
最高研发阶段:临床三期
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