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PCSK9 [Biotinylated] : LDL R Inhibitor Screening ELISA Assay Pair

Catalog Components Size (96 tests) Size (480 tests)
A006-214 Human LDL R 35 μg 160 μg
A007-214 Biotinylated Human PCSK9 10 μg 10 μg
A003-214 Streptavidin-HRP 10 μg 10 μg
PC9-NA003 Anti-PCSK9 Neutralizing Antibody 40 μg 200 μg

Background/背景

PCSK9 is a crucial protein in the regulation of plasma cholesterol homeostasis. It binds low density lipoprotein receptor (LDL R) to enhance the degradation of LDL R. Therefore, inhibition of PCSK9 has been considered a promising strategy to prevent the receptor from being degraded and promote removal of LDL cholesterol from circulation.

Application/应用

This pair is useful for screening for inhibitors of human PCSK9 binding to human LDL R.

Reconstitution/重构方法

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage/存储

Upon receipt, please store all items at -70℃.

After reconstitution, the stock solution should be kept at -70℃.

It is recommended not to freeze thaw more than 3 times.

No activity loss is observed after storage at:

  • Room temperature (RT) for 1 month in lyophilized state;
  • -20℃ for 1 year in lyophilized state;
  • -70℃ for 1 year under sterile conditions after reconstitution;

Assay Principles/原理

This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of new PCSK9 pathway inhibitors. This assay employs a simple colorimetric ELISA platform, which measures the binding between immobilized human LDL R and in-house developed biotinylated PCSK9 protein. This product is uniquely suitable for rapid high-throughput screening of putative PCSK9 inhibitors. Briefly, we provide you with a biotinylated human PCSK9 protein, a human LDL R protein, an anti-PCSK9 neutralizing antibody (as method verified Reference), and Streptavidin-HRP reagent. Your experiment will include 4 simple steps:

  • Coat the plate with human LDL R.
  • Add your molecule of interest to the plates.
  • Add human PCSK9-Biotin to the plates.
  • Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

Finally, the ability of your compound to inhibit PCSK9: LDL R binding will be determined by comparing OD readings among different experimental groups.

 

Typical Data/典型数据

Biotinylated Human FACS

Binding of Biotinylated Human PCSK9 to Immobilized Human LDL R in a Functional ELISA Assay.

Immobilized human LDL R protein at 3 μg/mL (100 μL/well) can bind biotinylated human PCSK9 with a linear range of 0.000625-0.02 μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points before curve fitting.

Biotinylated Human FACS

Inhibition of PCSK9 [Biotinylated] : LDL R Binding by Anti-PCSK9 Neutralizing Antibody.

Serial dilutions of anti-PCSK9 neutralizing antibody (Catalog # PC9-NA003) (1:2 serial dilutions, from 20 μg/mL to 0.04 μg/mL) were added into PCSK9: biotinylated LDL R binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points prior to log transformation and curve fitting.

 

前沿进展

货号/价格

EP-103-96tests / ¥6000

EP-103-480tests / ¥21500

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PCSK9靶点信息
英文全称:Proprotein convertase subtilisin kexin type 9
中文全称:前蛋白转化酶枯草杆菌蛋白酶Kexin-9
种类:Homo sapiens
上市药物数量:2详情
临床药物数量:22详情
最高研发阶段:批准上市
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