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Biotinylated Human ErbB3 / Her3 Protein, His,Avitag™

分子别名(Synonym)

ERBB3,HER3,LCCS2,MDA-BF-1,MGC88033,c-erbB3,erbB3-S,p180-ErbB3,p45-sErbB3,p85-sErbB3

表达区间及表达系统(Source)

Biotinylated Human ErbB3, His,Avitag (ER3-H82E6) is expressed from human 293 cells (HEK293). It contains AA Ser 20 - Thr 643 (Accession # P21860-1).

Predicted N-terminus: Ser 20

蛋白结构(Molecular Characterization)

Online(Ser 20 - Thr 643) P21860-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 72.4 kDa. The protein migrates as 95-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by reduced SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human ErbB3, His,Avitag (Cat. No. ER3-H82E6) SDS-PAGE gel

Biotinylated Human ErbB3, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human NRG1-beat 1 Protein at 2 μg/mL (100 μL/well) can bind Biotinylated Human ErbB3, His,Avitag (Cat. No. ER3-H82E6) with a linear range of 0.039-0.625 μg/mL (QC tested).

Protocol

背景(Background)

ErbB3,also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

 

前沿进展

货号/价格

ER3-H82E6-25ug / ¥3500

ER3-H82E6-200ug / ¥13800

开发建议
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联系电话:
400-682-2521(全国)
010-57737274(北京)
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运输方式
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技术支持邮箱:
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ErbB3靶点信息
英文全称:Receptor tyrosine-protein kinase erbB-3
中文全称:受体酪氨酸蛋白激酶erbB-3
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:24详情
最高研发阶段:临床三期
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