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Human Mucin-1 / MUC-1 (1036-1155) Protein, His Tag

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分子别名(Synonym)

Mucin 1,MUC1,CD227,EMA,H23AG,KL-6,MAM6,MUC-1,SEC,MUC-1,X,MUC1,ZD,PEM,PEMT,PUM,CA15-3,Episialin

表达区间及表达系统(Source)

Human Mucin-1 (1036-1155), His Tag (MU1-H52H5) is expressed from human 293 cells (HEK293). It contains AA Leu 1036 - Gly 1155 (Accession # P15941-1 ).

Predicted N-terminus: Leu 1036 (partial α chain) & Ser 1098 (partial β chain)

Request for sequence

蛋白结构(Molecular Characterization)

Mucin-1 Structure

This protein carries a polyhistidine tag at the C-terminus. The mature form of Mucin-1 is a non-covalent heterodimeric complex with the proteolytically cleaved partial α and partial β chain. Each partial α and partial β chain has a calculated MW of 7.2 kDa (partial α chain) and 8.2 kDa (partial β chain). The protein migrates as 13-15 kDa (partial α chain) and 10 kDa (partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mucin-1 SDS-PAGE

Human Mucin-1 (1036-1155), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Mucin-1 ELISA

Immobilized Human Mucin-1 (1036-1155), His Tag (Cat. No. MU1-H52H5) at 1 μg/mL (100 μL/well) can bind Anti-Mucin-1 Antibody, Mouse IgG1 with a linear range of 0.2-6 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-FACS

Mucin-1 FACS

2e5 of anti-Mucin-1 CAR-293 were stained with 100 μL of 0.1 μg/mL of Human Mucin-1, His Tag (Cat. No. MU1-H52H5) and negative control protein respectively, washed and then followed by PE anti-His Tag antibody and analyzed with FACS (Routinely tested).

Protocol

 

背景(Background)

Membrane mucins have several functions in epithelial cells including cytoprotection, extravasation during metastases, maintenance of luminal structure, and signal transduction. MUC17, contains an extended, repetitive extracellular glycosylation domain and a carboxyl terminus with two EGF-like domains, a SEA module domain, a transmembrane domain, and a cytoplasmic domain with potential serine and tyrosine phosphorylation sites. Interacts via its C-terminus with PDZK1 and this interaction appears important for proper localization. Probably plays a role in maintaining homeostasis on mucosal surfaces.

 

前沿进展

 
 
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Mucin-1靶点信息
英文全称:Mucin-1
中文全称:黏蛋白-1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:21详情
最高研发阶段:临床二期
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