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Cynomolgus B7-H2 / ICOSLG Protein, Fc Tag

分子别名(Synonym)

ICOSLG,B7-H2,B7H2,B7RP-1,B7RP1,CD275,GL50,ICOS-L,ICOSL,LICOS,ICOS ligand

表达区间及表达系统(Source)

Cynomolgus B7-H2, Fc Tag (B72-C525b) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Thr 256 (Accession # XP_005548617.1).

Predicted N-terminus: Asp 19

蛋白结构(Molecular Characterization)

Online(Asp 19 - Thr 256) XP_005548617.1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 53.0 kDa. The protein migrates as 60-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Cynomolgus B7-H2, Fc Tag (Cat. No. B72-C525b) SDS-PAGE gel

Cynomolgus B7-H2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human ICOS (C136S, C137S), His Tag (Cat. No. ICS-H52H6) at 5 μg/mL (100 μL/well) can bind Cynomolgus B7-H2, Fc Tag (Cat. No. B72-C525b) with a linear range of 0.1-4 ng/mL (QC tested).

Protocol

背景(Background)

ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.

 

前沿进展

货号/价格

B72-C525b-100ug / ¥3000

B72-C525b-1mg (500ug X 2) / ¥19800

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