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Human Siglec-10 Protein, Fc Tag

分子别名(Synonym)

SIGLEC10,MGC126774,PRO940,Siglec10,SLG2

表达区间及表达系统(Source)

Human Siglec-10, Fc Tag (SI0-H5253) is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).

Predicted N-terminus: Met 17

蛋白结构(Molecular Characterization)

Online(Met 17 - Thr 546) Q96LC7-1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 84.6 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Siglec-10, Fc Tag (Cat. No. SI0-H5253) SDS-PAGE gel

Human Siglec-10, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Siglec-10, Fc Tag (Cat. No. SI0-H5253) at 5 μg/mL (100 μL/well) can bind Anti-Human Siglec-10 MAb, human IgG with a linear range of 0.6-10 ng/mL (QC tested).

Protocol

背景(Background)

The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

 

前沿进展

货号/价格

SI0-H5253-100ug / ¥3500

SI0-H5253-1mg / ¥23100

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Siglec-10靶点信息
英文全称:Sialic acid-binding Ig-like lectin 10
中文全称:唾液酸结合免疫球蛋白样凝集素-10
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床二期
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