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 >  Protein>CLEC12A >CLA-H5266

Human CLEC12A / MICL / CLL-1 Protein, Fc Tag (MALS verified)

分子别名(Synonym)

CLEC12A,MICL,CLL-1,CLL1,DCAL2,DCAL-2,CD371

表达区间及表达系统(Source)

Human CLEC12A, Fc Tag (CLA-H5266) is expressed from human 293 cells (HEK293). It contains AA His 65 - Ala 265 (Accession # Q5QGZ9-2).

Predicted N-terminus: Pro

Request for sequence

蛋白结构(Molecular Characterization)

CLEC12A Structure

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 50.2 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CLEC12A SDS-PAGE

Human CLEC12A, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

CLEC12A SEC-MALS

The purity of Human CLEC12A, Fc Tag (Cat. No. CLA-H5266) is more than 90% and the molecular weight of this protein is around 125-140 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

CLEC12A ELISA

Immobilized Human CLEC12A, Fc Tag (Cat. No. CLA-H5266) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CLEC12A Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-FACS

CLEC12A FACS

2e5 of anti-CLEC12A CAR-293 cells were stained with 100μL of 3 μg/mL of Human CLEC12A, Fc Tag (Cat. No. CLA-H5266) and negative control protein respectively, washed and then followed by PE anti-human IgG Fc antibody and analyzed with FACS (Routinely tested).

Protocol

 

背景(Background)

CLEC12A (C-type lectin domain family 12 member A) is also known as CLL1, DCAL2, MICL. Clec12a is an inhibitory receptor for uric acid crystals that regulates inflammation in response to cell death. Cell surface receptor that modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases. Evidence of distinct disease propagating stem cells in myelodysplastic syndrome (MDS) has emerged in recent years. The role of CLEC12A in MDS, however, remains to be elucidated. Furthermore, CLEC12A has been proposed as a promising marker of leukaemic stem cells in AML.

 

前沿进展

 
 
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CLEC12A靶点信息
英文全称:C-Type lectin domain family 12 member A
中文全称:C型凝集素结构域家族12成员A
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:20详情
最高研发阶段:临床三期
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