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 >  Protein>Complement C2 >CO2-H52H7

Human Complement C2 Protein, His Tag (MALS verified) (active enzyme)

分子别名(Synonym)

C2,Complement C2,Complement Component C2

表达区间及表达系统(Source)

Human Complement C2, His Tag (CO2-H52H7) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Leu 752 (Accession # P06681-1 ).

Predicted N-terminus: Ala 21

Request for sequence

蛋白结构(Molecular Characterization)

Complement C2 Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 83.0 kDa. The protein migrates as 95-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Complement C2 SDS-PAGE

Human Complement C2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Complement C2 SEC-MALS

The purity of Human Complement C2, His Tag (Cat. No. CO2-H52H7) is more than 90% and the molecular weight of this protein is around 90-115 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB).The specific activity is >100 pmol/min/µg (QC tested).

Protocol

 

背景(Background)

C2 is a major histocompatibility complex class-III protein. Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase. The lectin (LP) and classical (CP) pathways are two of the three main activation cascades of the complement system. These pathways start with recognition of different pathogen- or danger-associated molecular patterns and include identical steps of proteolytic activation of complement component C4, formation of the C3 proconvertase C4b2, followed by cleavage of complement component C2 within C4b2 resulting in the C3 convertase C4b2a.

 

前沿进展

 
 
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Complement C2靶点信息
英文全称:Complement C2
中文全称:补体C2
种类:
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床二期
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