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Biotinylated Human IL-2 R gamma / CD132 Protein, His,Avitag™ (SPR verified)

分子别名(Synonym)

IL2RG,CD132,CIDX,IMD4,P64,SCIDX,SCIDX1,gammaC

表达区间及表达系统(Source)

Biotinylated Human IL-2 R gamma, His,Avitag (ILG-H85E8) is expressed from Baculovirus-Insect cells. It contains AA Leu 23 - Asn 254 (Accession # P31785-1).

Predicted N-terminus: Leu 23

蛋白结构(Molecular Characterization)

Online(Leu 23 - Asn 254) P31785-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 31.0 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human IL-2 R gamma, His,Avitag (Cat. No. ILG-H85E8) SDS-PAGE gel

Biotinylated Human IL-2 R gamma, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

Biotinylated Human IL-2 R gamma, His,Avitag (Cat. No. ILG-H85E8) immobilized on SA Chip can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 105 μM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

 

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Biotinylated Human IL-2 R gamma, His,Avitag (Cat. No. ILG-H85E8) inhibits the IL-2-dependent proliferation of Mo7e cells. The EC50 for this effect is 4.08-5.90 µg/mL in the presence of 10 µg/mL of human IL-2 R beta and 50 ng/mL of human IL-2 (Routinely tested).

Protocol

 

背景(Background)

IL-2R is a heterotrimeric protein binds and responds to the cytokine IL-2. Three distinct chains of IL-2R, termed as α, β and γ, which are non-covalently associated are identified. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ chain, along with the β subunit. The α chain of the IL-2R can bind to the β chain before receptor interaction with IL-2. The γ chain alone has a very weak affinity for IL-2, but after the ligand is bound to the α/β heterodimer, the γ chain becomes recruited to the complex to form a very stable macromolecular quaternary ligand/receptor complex. Interleukin-2 receptor subunit gamma (IL2RG), also known as cytokine receptor common subunit gamma, CD antigen CD132, gammaC, p64, which belongs to the type I cytokine receptor family or type 5 subfamily. IL2RG is located on the surface of immature blood-forming cells in bone marrow. Defects in IL2RG are the cause of severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell-negative (XSCID).

 

前沿进展

 
货号/价格

ILG-H85E8-25ug / ¥3500

ILG-H85E8-200ug / ¥13800

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