3CL-Mpro,3C-like main protease
2019-nCoV 3CL-Mpro, His Tag (3CO-C51H5) is expressed from E.coli cells. It contains AA Ser 1 - Gln 306 (Accession # PDB: 6LU7_A).
Predicted N-terminus: Ser 1
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 35.7 kDa.
Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 100 mM NaCl, 2 mM TCEP, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
The Main protease (M pro) is a key enzyme for 2019-nCoV replication. It is also encoded by the polypeptide and responsible for processing the polypeptide into functional proteins. The M pro has similar cleavage-site specificity to that of picornavirus 3C protease (3C pro), thus is also known as 3C 3C-like main protease (3CL Mpro).