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SARS-CoV-2 (COVID-19) S protein (R683A, R685A), His Tag, active trimer (MALS verified)

分子别名(Synonym)

S protein,Spike glycoprotein,S glycoprotein,COVID-19

表达区间及表达系统(Source)

SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (SPN-C52H8) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (R683A, R685A)) trimer Design.

Predicted N-terminus: Val 16

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 138.0 kDa. The protein migrates as 160-230 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) SDS-PAGE gel

SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) MALS images

The purity of SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) was more than 80% and around 550-660 kDa verified by SEC-MALS.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human ACE2, Fc,Avitag (Cat. No. AC2-H82F9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) with a linear range of 10-40 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) at 2 μg/mL (100 μL/well) can bind Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) with a linear range of 4-63 ng/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.2441-7.8125 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human ACE2, Fc Tag (Cat. No. AC2-H5257) Captured on CM5 chip via anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) with an affinity constant of 35.3 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

前沿进展

货号/价格

SPN-C52H8-50ug / ¥4500

SPN-C52H8-500ug (100ug X 5) / ¥28000

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