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Human BAFF / TNFSF13B / CD257 Protein, His,Flag Tag, active trimer (MALS verified)

分子别名(Synonym)

TNFSF13B,BAFF,BLYS,CD257,DTL,TALL1,THANK,TNFSF20,ZTNF4,TALL-1

表达区间及表达系统(Source)

Human BAFF, His,Flag Tag, active trimer (BAF-H52D4) is expressed from human 293 cells (HEK293). It contains AA Glu 140 - Leu 285 (Accession # AAH20674.1) trimer Design.

Predicted N-terminus: His

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

The protein has a calculated MW of 52.5 kDa. The protein migrates as 55-60 kDa under non-reducing (NR) condition (SDS-PAGE) due to Glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH 6.0. Normally Trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human BAFF, His,Flag Tag, active trimer (Cat. No. BAF-H52D4) SDS-PAGE gel

Human BAFF, His,Flag Tag, active trimer on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human BAFF, His,Flag Tag, active trimer (Cat. No. BAF-H52D4) MALS images

The purity of Human BAFF, His,Flag Tag, active trimer (Cat. No. BAF-H52D4) was more than 90% and around 50-65 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human BAFFR, Fc Tag (Cat. No. BAR-H5257) at 2 μg/mL (100 μL/well) can bind Human BAFF, His,Flag Tag, active trimer (Cat. No. BAF-H52D4) with a linear range of 4-63 ng/mL when detected by Monoclonal anti FLAG (HRP) antibody (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human BCMA, Fc Tag (Cat. No. BC7-H5254) at 2 μg/mL (100 μL/well) can bind Human BAFF, His,Flag Tag, active trimer (Cat. No. BAF-H52D4) with a linear range of 2-16 ng/mL when detected by Monoclonal anti FLAG (HRP) antibody (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized Human TACI/TNFRSF13B, Fc Tag (Cat. No. TAI-H5256) at 2 μg/mL (100 μL/well) can bind BAFF, His,Flag Tag, active trimer (Cat. No. BAF-H52D4) with a linear range of 4-31 ng/mL when detected by Monoclonal anti FLAG (HRP) antibody(Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized Human BAFF, His,Flag Tag, active trimer (Cat. No. BAF-H52D4) at 2 μg/mL (100 μL/well) can bind Human TACI/TNFRSF13B, Fc Tag (Cat. No. TAI-H5256) with a linear range of 2-39 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human BAFFR Protein, Fc Tag (Cat. No. BAR-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human BAFF Protein, His,Flag Tag (Cat. No. BAF-H52D4) with an affinity constant of 5.54 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

B-cell activating factor (BAFF) is also known as tumor necrosis factor ligand superfamily member 13B , TNFSF13B, BAFF, B Lymphocyte Stimulator (BLyS) , cluster of differentiation 257 (CD257), DTL, TNF- and APOL-related leukocyte expressed ligand (TALL-1), THANK, TNFSF20, ZTNF4, and is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it. These receptors are expressed mainly on mature B lymphocytes (TACI is also found on a subset of T-cells and BCMA on plasma cells). TACI binds worst since its affinity is higher for a protein similar to BAFF, called a proliferation-inducing ligand (APRIL). BCMA displays an intermediate binding phenotype and will work with either BAFF or APRIL to varying degrees. Signaling through BAFF-R and BCMA stimulates B lymphocytes to undergo proliferation and to counter apoptosis. All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors, although BAFF has been known to be active as either a hetero- or homotrimer. BAFF acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells. 

 

前沿进展

 
货号/价格

BAF-H52D4-100ug / ¥3500

BAF-H52D4-1mg (500ug X 2) / ¥24000

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BAFF靶点信息
英文全称:B-cell-activating factor/B lymphocyte stimulator
中文全称:B细胞活化因子/B淋巴细胞刺激因子
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:11详情
最高研发阶段:批准上市
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