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SARS-CoV-2 (COVID-19) S protein (R683A, R685A), His Tag, active trimer (MALS verified)

分子别名(Synonym)

S protein,Spike glycoprotein,S glycoprotein,COVID-19

表达区间及表达系统(Source)

SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (MALS verified) (SPN-C52H2) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1(R683A, R685A)) trimer Design.

Predicted N-terminus: Val 16

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 138.0 kDa. The protein migrates as 180-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH5.5.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied as sterile liquid solution and shipped frozen with dry ice.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

电泳(SDS-PAGE)

SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (MALS verified) (Cat. No. SPN-C52H2) SDS-PAGE gel

SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (MALS verified) (Cat. No. SPN-C52H2) MALS images

The purity of SARS-CoV-2 (COVID-19) S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H2) was more than 85% and around 550-680 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (MALS verified) (Cat. No. SPN-C52H2) with a linear range of 0.9-31 ng/mL (QC tested).

Protocol

背景(Background)

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

前沿进展

货号/价格

SPN-C52H2-200ug / 询价

SPN-C52H2-1mg / 询价

SPN-C52H2-10mg (5mg X 2) / 询价

SPN-C52H2-100mg / 询价

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