膜杰作
Star Staining
描述（Description）
Binding assay by FACS and cell based ELISA.
应用说明（Application）
Human PD-L1, GFP Tag-HEK293 cells stably expressing exogenous human PD-L1 (Programmed cell death 1 ligand 1, CD274, B7-H1, PDCD1L1, PDCD1LG1, AA sequence : Leu 19 - Leu 238, Protein Acc.# : Q9NZQ7-1). At the same time, GFP protein as a marker was expressed in HEK239/hPD-L1 cells for screening positive cells by FACS.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
G418 (0.5mg/mL)
培养基（Culture Medium）
DMEM medium +10% FBS
冻存液（Freeze Medium）
20% FBS+10% DMSO+70% DMEM medium
装量（Quantity）
1 vial contains ~ 5x10^6 cells in 1 ml of 10% DMSO in (DMEM+22%FBS).
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
活性（Bioactivity）
Flow Cytometry assay shows that PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) can bind to 293T cells overexpressing human PD-L1. 1 μl stock solution per million cells (Routinely tested).
Protocol
FACS analysis shows that the binding of PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) to 293T overexpressing PD-L1 was inhibited by increasing concentration of neutralizing anti- human PD-L1 antibody. The IC50 is 0.29 μg/ml (Routinely tested).
Protocol
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
