|TAS017-C01||SARS-CoV-2 Spike protein RBD||10ug|
|TAS017-C02||Anti-SARS-CoV-2 Antibody (Control, IgG4)||10ug|
|TAS017-C03||HRP-Mouse anti-Human IgG4 HRP||10ug|
The ongoing SARS-COV-2 pandemics has sickened 3.7 million people around the world, claiming over 1,000,000 lives. To deal with this global public health crisis, unprecedented efforts are being made to study the virus, SARS-CoV-2. At present, there are 193 new crown vaccine projects registered with the World Health Organization are being developed. To support these studies, ACRO has developed Anti-SARS-CoV-2 subtype antibody Serologic Assay kit including:IgG1, IgG2, IgG3, IgG4.
This kit is developed for serologic test for IgG4 titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃.
It is recommended not to freeze thaw more than 3 times.
This product is stable under storage conditions:
12 months in sealed state;
Used within 3 months after opening.
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike protein RBD. The Kit consists of High-bind Plate, Spike protein RBD, an Anti-SARS-CoV-2 Antibody (Control, IgG4), an HRP-Anti-Human IgG4 secondary antibodyr.
Your experiment will include 6 simple steps:
a) Coat the plate with SARS-CoV-2 Spike protein RBD.
b) Wash the plate with universal ELISA buffer and block the plate with the blocking buffer.
c) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Blocking / Dilution Buffer.
d) Add diluted Secondary antibody HRP-Mouse-Anti-Human IgG4 to the plate. The Secondary antibody is diluted by Blocking / Dilution Buffer.
e) Wash the plate and add TMB or other colorimetric HRP substrate.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.