Human GDF-2, Tag Free (GD2-H5211) is expressed from human 293 cells (HEK293). It contains AA Ser 320 - Arg 429 (Accession # Q9UK05-1).
Predicted N-terminus: Ser 320
This protein carries no "tag".
The protein has a calculated MW of 12.1 kDa. The protein migrates as 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in ACN, TFA in Water. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human GDF-2, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human GDF-2, Tag Free (Cat. No. GD2-H5211) at 1 μg/mL (100 μL/well) can bind Human ALK-1, His Tag (Cat. No. AL1-H5227) with a linear range of 2-16 ng/mL (QC tested).