Integrin alpha D beta 2,ITGAD & ITGB2
Human ITGAD&ITGB2 Heterodimer Protein, His Tag&Tag Free (IT2-H52W5) is expressed from human 293 cells (HEK293). It contains AA Phe 18 - Asn 1099 (ITGAD) & Gln 23 - Asn 700 (ITGB2) (Accession # Q13349-1(ITGAD) & P05107-1(ITGB2)).
Human ITGAD & ITGB2 Heterodimer Protein, produced by co-expression of ITGAD and ITGB2, has a calculated MW of 125 kDa (ITGAD) & 80.1 kDa (ITGB2). Subunit ITGAD is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB2 contains no tag but a basic tail at the C-terminus.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. May play a role in the atherosclerotic process such as clearing lipoproteins from plaques and in phagocytosis of blood-borne pathogens, particulate matter, and senescent erythrocytes from the blood. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed:18587400). Alpha D beta 2, selectively binds ICAM-3 and does not appear to bind ICAM-1. In addition, a soluble form of alpha D beta 2 binds VCAM-1 with greater efficiency relative to ICAM-3. The I domain of alpha D contains a binding site for VCAM-1 since recombinant alpha D I domain binds specifically to VCAM-1.