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Human CD155 / PVR Protein, Fc Tag

分子别名(Synonym)

PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5

表达区间及表达系统(Source)

Human CD155, Fc Tag (CD5-H5251) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Asn 343 (Accession # AAH15542).

Predicted N-terminus: Trp 21

蛋白结构(Molecular Characterization)

Unconjugated(Trp 21 - Asn 343) AAH15542

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 61.7 kDa. The protein migrates as 95-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD155, Fc Tag (Cat. No. CD5-H5251) SDS-PAGE gel

Human CD155, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD155, Fc Tag (Cat. No. CD5-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TIGIT, His Tag (Cat. No. TIT-H82E5) with a linear range of 5-78 ng/mL (QC tested).

点击申请Protocol

活性(Bioactivity)-FACS

Biotinylated Human FACS

FACS assay shows that recombinant Human CD155, Fc Tag (Cat .No. CD5-H5251) can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 μg/ml (Routinely tested).

点击申请Protocol

Biotinylated Human FACS

FACS analysis shows that the binding of Human CD155, Fc Tag to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 μg/ml. The IC50 is 0.09422 μg/ml (Routinely tested).

点击申请Protocol

 

文献引用(Citations)

背景(Background)

CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. The fulllength (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N terminal V type and two C2 type Ig like domains. CD155 is a transmembrane protein with 3 extracellular immunoglobulin-like domains, D1-D3, where D1 is recognized by the virus. Low resolution structures of CD155 complexed with poliovirus have been obtained using electron microscopy while a high resolution structures of theectodomain D1 and D2 of CD155 were solved by x-ray crystallography.

 

前沿进展

货号/价格

CD5-H5251-100ug / ¥2800

CD5-H5251-1mg (250ug x 4) / ¥18000

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