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Human CD40 / TNFRSF5 Protein, Fc Tag (HPLC-verified)

分子别名(Synonym)

CD40,Bp50,CDW40,MGC9013,TNFRSF5,p50

表达区间及表达系统(Source)

Human CD40, Fc Tag (HPLC-verified) (CD0-H5253) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # NP_001241.1).

Predicted N-terminus: Glu 21

蛋白结构(Molecular Characterization)

Unconjugated(Glu 21 - Arg 193) NP_001241.1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 45.3 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD40, Fc Tag (HPLC-verified) (Cat. No. CD0-H5253) SDS-PAGE gel

Human CD40, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC

Human CD40, Fc Tag (HPLC-verified) (Cat. No. CD0-H5253) HPLC images

The purity of Human CD40, Fc Tag (HPLC-verified) (Cat. No. CD0-H5253) was greater than 90% as determined by SEC-HPLC.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD40 Ligand, His Tag (Cat. No. CDL-H5248) at 5 μg/mL (100 μL/well) can bind Human CD40, Fc Tag (HPLC-verified) (Cat. No. CD0-H5253) with a linear range of 0.039-1.25 μg/mL (QC tested).

点击申请Protocol

活性(Bioactivity)-FACS

Biotinylated Human FACS

FACS assay shows that recombinant Human CD40, Fc Tag (HPLC-verified) (Cat. No. CD0-H5253) can bind to 293T cells overexpressing human CD40L, and the binding is inhibited by neutralizing Anti-Human CD40 MAb. The concentration of CD40 and Anti-Human CD40 MAb used are 0.03 μg/ml and 0.3 μg/ml respectively.

点击申请Protocol

 

背景(Background)

CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. The extracellular domain has the cysteinerich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 molecules,which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching, as well as an inability to mount an antibody-specific immune response, and a lack of germinal center formation.

 

前沿进展

货号/价格

CD0-H5253-100ug / ¥2900

CD0-H5253-1mg / ¥19800

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CD40靶点信息
英文全称:Tumor necrosis factor receptor superfamily member 5
中文全称:肿瘤坏死因子受体超家族成员5
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:17详情
最高研发阶段:临床二期
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