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 >  Protein>Fc epsilon RI alpha >FCA-H5228

Human Fc epsilon RI alpha Protein, His Tag (MALS verified)

分子别名(Synonym)

FCER1A,FCE1A,FcERI

表达区间及表达系统(Source)

Human Fc epsilon RI alpha, His Tag (FCA-H5228) is expressed from human 293 cells (HEK293). It contains AA Val 26 - Gln 205 (Accession # P12319-1 ).

Predicted N-terminus: Val 26

Request for sequence

蛋白结构(Molecular Characterization)

Fc epsilon RI alpha Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 22.9 kDa. The protein migrates as 37-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Fc epsilon RI alpha SDS-PAGE

Human Fc epsilon RI alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Fc epsilon RI alpha SEC-MALS

The purity of Human Fc epsilon RI alpha, His Tag (Cat. No. FCA-H5228) is more than 90% and the molecular weight of this protein is around 37-55 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Fc epsilon RI alpha ELISA

Immobilized Immunoglobulin E, Human Plasma at 5 μg/mL (100 μL/well) can bind Human Fc epsilon RI alpha, His Tag (Cat. No. FCA-H5228) with a linear range of 0.4-3 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-BLI

Fc epsilon RI alpha BLI

Loaded Human Fc epsilon RI alpha, His Tag (Cat. No. FCA-H5228) on AR2G Biosensor, can bind Immunoglobulin E, Human Plasma with an affinity constant of 2.71 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

High affinity immunoglobulin epsilon receptor subunit alpha (FCER1A) is also known as Fc-epsilon RI-alpha (FcERI), IgE Fc receptor subunit alpha, FCE1A. FCER1A contains two Ig-like (immunoglobulin-like) domains. FCER1A binds to the Fc region of immunoglobulins epsilon and is a high affinity receptor. FCER1A is responsible for initiating the allergic response, which binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. FCER1A plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma.

 

前沿进展

 
 
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Fc epsilon RI alpha靶点信息
英文全称:IgE Fc receptor, alpha-subunit
中文全称:IgE-Fc片段受体α亚基
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:0详情
最高研发阶段:批准上市
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