Human CD64, His Tag (SPR & BLI verified) (FCA-H52H2) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Pro 288 (Accession # AAH32634).
Predicted N-terminus: Gln 16
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 32.5 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human CD64, His Tag (SPR & BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Ipilimumab at 5 μg/mL (100 μL/well) can bind Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H2) with a linear range of 0.05-2 ng/mL (QC tested).
Immobilized Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H2) on CM5 Chip via anti-His antibody, can bind Herceptin with an affinity constant of 5.45 nM as determined in a SPR assay (Biacore T200) (QC tested).
Loaded Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H2) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 13 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Simonov V, et al
Journal: Biologicals 43480
Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.