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 >  Protein>Galectin-9 >LG9-H5244

Human Galectin-9 / LGALS9 Protein, His Tag

分子别名(Synonym)

Galectin-9,LGALS9,Ecalectin

表达区间及表达系统(Source)

Human Galectin-9, His Tag (LG9-H5244) is expressed from human 293 cells (HEK293). It contains AA Ala 2 - Thr 323 (Accession # O00182-2 ).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

Galectin-9 Structure

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 36.6 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 20 mM MOPS, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Galectin-9 SDS-PAGE

Human Galectin-9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-SPR

Galectin-9 SPR

Human TIM-3 (22-200) Protein, Fc Tag (Cat. No. TM3-H5258) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human Galectin-9, His Tag (Cat. No. LG9-H5244) with an affinity constant of 57 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

Galectin-9 (LGALS9) is also known as tumor antigen HOM-HD-21 and ecalectin. Galectins are a family of proteins defined by their binding specificity for β-galactoside sugars, such as N-acetyllactosamine (Galβ1-3GlcNAc or Galβ1-4GlcNAc), which can be bound to proteins by either N-linked or O-linked glycosylation. As for Galectin-9, it has high affinity for the Forssman pentasaccharide, and it is also the ligand for HAVCR2/TIM3. Forthermore, Galectin-9 stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth.

文献引用(Citations)

 

前沿进展

 
 
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Galectin-9靶点信息
英文全称:Galactoside-binding lectin Galectin 9
中文全称:半乳糖凝集素-9
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床二期
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