登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>HVEM >HVM-H52E9

Human HVEM / TNFRSF14 Protein, His Tag (MALS verified)

分子别名(Synonym)

TNFRSF14,ATAR,HVEA,HVEM,LIGHTR,TR2,CD270

表达区间及表达系统(Source)

Human HVEM, His Tag (HVM-H52E9) is expressed from human 293 cells (HEK293). It contains AA Leu 39 - Val 202 (Accession # Q92956-1 ).

Predicted N-terminus: Leu 39

Request for sequence

蛋白结构(Molecular Characterization)

HVEM Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 19.2 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

HVEM SDS-PAGE

Human HVEM, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

HVEM SEC-MALS

The purity of Human HVEM, His Tag (Cat. No. HVM-H52E9) is more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

HVEM ELISA

Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Human BTLA, Fc Tag (Cat. No. BTA-H5256) with a linear range of 0.01-0.313 μg/mL (QC tested).

Protocol

HVEM ELISA

Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. LIT-H5256) at 2 μg/mL (100 μL/well) can bind Human HVEM, His Tag (Cat. No. HVM-H52E9) with a linear range of 0.01-0.156 μg/mL (Routinely tested).

Protocol

 

背景(Background)

Herpesvirus entry mediator (HVEM) is also known as TNFRSF14, TR2 (TNF receptorlike molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.

文献引用(Citations)

 

前沿进展

 
 
货号/价格
产品推荐
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
HVEM靶点信息
英文全称:Tumor necrosis factor receptor superfamily member 14
中文全称:肿瘤坏死因子受体超家族成员14
种类:
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:临床前
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定