Human OX40 Ligand, His Tag (active trimer) (HPLC-verified) (OXL-H52Q8) is expressed from human 293 cells (HEK293). It contains AA Gln 51 - Leu 183 (Accession # NP_003317).
Predicted N-terminus: His
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 16.9 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human OX40 Ligand, His Tag (active trimer) (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
A SEC-HPLC analysis showing over 90% of Human OX40 Ligand, His Tag (active trimer) (HPLC-verified) (Cat. No. OXL-H52Q8) present as active trimers with a molecular mass around 90 kDa.
Immobilized Biotinylated Human OX40, Fc,Avitag (Cat. No. OX0-H82F7) at 2 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Human OX40 Ligand, His Tag (active trimer) (HPLC-verified) (Cat. No. OXL-H52Q8) with a linear range of 5-39 ng/mL (QC tested).
Immobilized Human OX40 Ligand, His Tag (active trimer) (HPLC-verified) (Cat. No. OXL-H52Q8) at 2 μg/mL (100 μL/well) can bind Biotinylated Human OX40, Fc,Avitag (Cat. No. OX0-H82F7) with a linear range of 10-78 ng/mL (Routinely tested).
FACS assay shows that Human OX40 Ligand, His Tag (active trimer) (HPLC-verified) (Cat. No. OXL-H52Q8) can bind to 293T cell overexpressing human OX40. The concentration of OX40 Ligand used is 0.1 μg/mL (Routinely tested).
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.