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Human PD-L2 / B7-DC Protein, Fc Tag (HPLC-verified)

分子别名(Synonym)

PDL2,PD-L2,Butyrophilin B7-DC,CD273,PDCD1 ligand 2,PDCD1L2,PDCD1LG2

表达区间及表达系统(Source)

Human PD-L2, Fc Tag (HPLC-verified) (PD2-H5251) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679).

Predicted N-terminus: Leu 20

蛋白结构(Molecular Characterization)

Unconjugated(Leu 20 - Pro 219) AAI13679

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 49.2 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human PD-L2, Fc Tag (HPLC-verified) (Cat. No. PD2-H5251) SDS-PAGE gel

Human PD-L2, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC

Human PD-L2, Fc Tag (HPLC-verified) (Cat. No. PD2-H5251) HPLC images

The purity of Human PD-L2, Fc Tag (HPLC-verified)(Cat.# PD2-H5251) was greater than 90% as determined by SEC-HPLC.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 200 μg/mL (100 μL/well) can bind Human PD-L2, Fc Tag (Cat. No. PD2-H5251) with a linear range of 0.156-0.625 μg/mL (QC tested).

点击申请Protocol

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L2, Fc Tag (HPLC-verified) (Cat. No. PD2-H5251) with an EC50 of 0.018 μg/ml (Routinely tested).

点击申请Protocol

 

活性(Bioactivity)-FACS

Biotinylated Human FACS

Flow Cytometry assay shows that recombinant Human PD-L2, Fc Tag (Cat. No. PD2-H5251) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 1 μg/mL (Routinely tested).

点击申请Protocol

 

文献引用(Citations)

背景(Background)

Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

 

前沿进展

货号/价格

PD2-H5251-100ug / ¥2600

PD2-H5251-1mg / ¥17000

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