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 >  Protein>TIGIT >TIT-H5254

Human TIGIT Protein, Fc Tag (MALS verified)

分子别名(Synonym)

TIGIT,VSIG9,VSTM3

表达区间及表达系统(Source)

Human TIGIT, Fc Tag (TIT-H5254) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).

Predicted N-terminus: Met 22

Request for sequence

蛋白结构(Molecular Characterization)

TIGIT Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 39.7 kDa. The protein migrates as 45-54 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

TIGIT SDS-PAGE

Human TIGIT, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

TIGIT SEC-MALS

The purity of Human TIGIT, Fc Tag (Cat. No. TIT-H5254) is more than 90% and the molecular weight of this protein is around 85-100 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

TIGIT ELISA

Immobilized Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) at 5 μg/mL (100 μL/well) can bind Human TIGIT, Fc Tag (Cat. No. TIT-H5254) with a linear range of 0.6-10 ng/mL (QC tested).

Protocol

TIGIT ELISA

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human TIGIT, Fc Tag (Cat. No. TIT-H5254): Biotinylated Human CD155, Fc,Avitag (Cat. No. CD5-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.55834 μg/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

TIGIT BLI

Loaded Human TIGIT, Fc Tag (Cat. No. TIT-H5254) on Protein A Biosensor, can bind Human CD155, His Tag (Cat. No. CD5-H5223) with an affinity constant of 0.98 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性(Bioactivity)-FACS

TIGIT FACS

FACS assay shows that recombinant Human TIGIT, Fc Tag (Cat. No. TIT-H5254) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.1 μg/mL (Routinely tested).

Protocol

 

背景(Background)

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

 

前沿进展

 
 
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TIGIT靶点信息
英文全称:T cell immunoglobulin and ITIM domains
中文全称:T细胞免疫球蛋白ITIM结构域
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:38详情
最高研发阶段:临床三期
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