搜索
 >  Fc受体蛋白>Fc gamma RIII / CD16 >Mouse Fc gamma RIII / CD16 Protein

Mouse Fc gamma RIII / CD16 Protein

分子别名(Synonym)

FCGR3

表达区间及表达系统(Source)

Mouse CD16 (CDA-M52H8) is expressed from human 293 cells (HEK293). It contains AA Leu 32 - Thr 215 (Accession # Q5D5I8).

Predicted N-terminus: Leu 32

蛋白结构(Molecular Characterization)

Unconjugated(Leu 32 - Thr 215) Q5D5I8

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 22.95 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by reduced SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse CD16 (Cat. No. CDA-M52H8) SDS-PAGE gel

Mouse CD16 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized OKT3 at 10 μg/mL (100 μL/well) can bind Mouse CD16 (Cat. No. CDA-M52H8) with a linear range of 78-625 ng/mL (QC tested).

点击申请Protocol

背景(Background)

CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody­dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

 

前沿进展

货号/价格

CDA-M52H8-100ug / ¥2800

CDA-M52H8-1mg (500ug X 2) / ¥18000

产品定制
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com

消息提示

请输入您的联系方式,再点击提交!

确定