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Rabbit CTLA-4 / CD152 Protein, Fc Tag

分子别名(Synonym)

CTLA4,CD152

表达区间及表达系统(Source)

Rabbit CTLA-4, Fc Tag (CT4-R5250) is expressed from human 293 cells (HEK293). It contains AA Lys 36 - Asp 161 (Accession # P42072).

Predicted N-terminus: Lys 36

蛋白结构(Molecular Characterization)

Online(Lys 36 - Asp 161) P42072

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 40.3 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Rabbit CTLA-4, Fc Tag (Cat. No. CT4-R5250) SDS-PAGE gel

Rabbit CTLA-4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human B7-1, mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) at 5 μg/mL (100 μL/well)can bind Rabbit CTLA-4, Fc Tag (Cat. No. CT4-R5250) with a linear range of 0.1-0.8 ng/mL (QC tested).

Protocol

Biotinylated Human SPR

Immobilized Human B7-2, His Tag (Cat. No. CD6-H5223) at 5 μg/mL (100 μL/well)can bind Rabbit CTLA-4, Fc Tag (Cat. No. CT4-R5250) with a linear range of 5-78 ng/mL (Routinely tested).

Protocol

背景(Background)

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

 

前沿进展

货号/价格

CT4-R5250-100ug / ¥3500

CT4-R5250-1mg (500ug X 2) / ¥22000

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CTLA-4靶点信息
英文全称:Cytotoxic T-Lymphocyte-Associated Antigen 4
中文全称:细胞毒性T淋巴细胞相关抗原4
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:30详情
最高研发阶段:批准上市
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