膜杰作
Star Staining
分子别名（Synonym）
DNAM1,CD226,PTA1
表达区间及表达系统（Source）
Biotinylated Human DNAM-1, Fc,Avitag (DN1-H82F9) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Asn 247 (Accession # NP_006557).
Predicted N-terminus: Glu 19
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 54.4 kDa. The protein migrates as 66-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human DNAM-1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Rhesus macaque CD155, His Tag (Cat. No. CD5-R52H5) at 5 μg/mL (100 μL/well) can bind Biotinylated Human DNAM-1, Fc,Avitag (Cat. No. DN1-H82F9) with a linear range of 10-156 ng/mL (QC tested).
Protocol
活性（Bioactivity）-FACS
FACS assay shows Biotinylated Human DNAM-1, Fc,Avitag (Cat. No. DN1-H82F9) can bind to 293T cell overexpressing human CD155. The concentration of DNAM-1 is 0.03 μg/mL (Routinely tested).
Protocol
背景（Background）
DX accessory molecule 1 (DM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DM-1 with antibodies causes cellular activation. Furthermore, DM-1 can interact with PVR and PVRL2.
