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Human TIGIT Protein, Mouse IgG2a Fc Tag, low endotoxin

分子别名(Synonym)

TIGIT,VSIG9,VSTM3

表达区间及表达系统(Source)

Human TIGIT, Mouse IgG2a Fc tag (TIT-H5253) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # AAI01290).

Predicted N-terminus: Met 22

蛋白结构(Molecular Characterization)

Unconjugated(Met 22 - Pro 141) AAI01290

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 40.1 kDa. The protein migrates as 42-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human TIGIT, Mouse IgG2a Fc tag (Cat. No. TIT-H5253) SDS-PAGE gel

Human TIGIT, Mouse IgG2a Fc tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD155, Fc Tag (Cat. No. CD5-H5251) at 5μg/mL (100 μL/well) can bind Human TIGIT, mouse IgG2a Fc tag (Cat. No. TIT-H5253) with a linear range of 3.9-62.5 ng/mL (QC tested).

点击申请Protocol

活性(Bioactivity)-FACS

Biotinylated Human FACS

FACS assay shows that recombinant Human TIGIT Protein, Mouse IgG2a Fc Tag (Cat. No. TIT-H5253) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.03 μg/ml (Routinely tested).

点击申请Protocol

 

背景(Background)

TIGIT (also called T cell immunoreceptor with Ig and ITIM domains) is one newly discovered immune receptor on some percentage of T cells and Natural Killer Cells(NK). It is also identified as WUCAM and Vstm3. TIGIT could bind to CD155(PVR) on dendritic cells(DCs), macrophages, etc. with high affinity, and also to CD112(PVRL2) with lower affinity. TIGIT expressing CD8+ T cells has been shown to be expanded and associated with clinical markers of HIV disease progression in a diverse group of HIV infected individuals. TIGIT and PD-1 has been shown to be over expressed on tumor antigen-sepcific (TA-specific) CD8+ T cells and CD8+ tumor infiltrating lymphocytes (TILs) from individuals with melanoma. Blockade of TIGIT and PD-1 led to increased cell proliferation, cytokine production, and degranulation of TA-specific CD8+ T cells and TIL CD8+ T cells.

 

前沿进展

货号/价格

TIT-H5253-100ug / ¥3500

TIT-H5253-1mg (500ug x 2) / ¥21000

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TIGIT靶点信息
英文全称:T cell immunoglobulin and ITIM domains
中文全称:T细胞免疫球蛋白ITIM结构域
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床一期
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