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 >  Protein>IL-1 beta >ILB-H4110

Human IL-1 beta / IL-1F2 Protein, premium grade

GMP Version Available: GMP Human IL-1 beta Protein   

分子别名(Synonym)

IL1B,IL-1BETA,IL1F2,IL-1β

表达区间及表达系统(Source)

Human IL-1 beta, premium grade (ILB-H4110) is expressed from E. coli cells. It contains AA Ala 117 - Ser 269 (Accession # P01584-1).

Predicted N-terminus: Met

Human IL-1 beta, premium grade (ILB-H4110), designed for preclinical stage, has the same activity and performance with GMP Human IL-1 beta (GMP-ILBH16), which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.

Request for sequence

蛋白结构(Molecular Characterization)

IL-1 beta Structure

This protein carries no "tag".

The protein has a calculated MW of 17.5 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 0.02 EU per μg by the LAL method.

无菌(Sterility)

The sterility testing was performed by membrane filtration method.

支原体(Mycoplasma)

Negative.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-1 beta SDS-PAGE

Human IL-1 beta, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

IL-1 beta SEC-MALS

The purity of Human IL-1 beta, premium grade (Cat. No. ILB-H4110) is more than 95% and the molecular weight of this protein is around 15-23 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-Bioactivity CELL BASE

IL-1 beta CELL

Human IL-1 beta, premium grade (Cat. No. ILB-H4110) stimulates secretion of IFN-γ by NK-92 human natural killer lymphoma cells stimulated with 0.5 ng/mL Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (Cat. No. IL2-H4210). The specific activity of Human IL-1 beta, premium grade is > 7.0 × 10^7 IU/mg, which is calibrated against human IL-1 beta WHO International Standard (NIBSC code: 86/680) (Routinely tested).

Protocol

 

活性(Bioactivity)-ELISA

IL-1 beta ELISA

Immobilized Human IL-1 beta, premium grade (Cat. No. ILB-H4110) at 10 μg/mL (100 μL/well) can bind Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) with a linear range of 8-63 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-SPR

IL-1 beta SPR

Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, premium grade (Cat. No. ILB-H4110) with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

Interleukin-1 beta (IL-1β) is also known as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene. IL-1β precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1 beta.
IL1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1α and IL1β are regulated independently, they bind to the same receptor and exert identical biological effects.
IL-1β is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

 

前沿进展

 
 
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IL-1 beta靶点信息
英文全称:Interleukin-1 beta
中文全称:白细胞介素-1β
种类:Homo sapiens
上市药物数量:7详情
临床药物数量:14详情
最高研发阶段:批准上市
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