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ActiveMax® Recombinant Human IL-4

分子别名(Synonym)

IL4,BCGF1,BSF1

表达区间及表达系统(Source)

Human IL-4, Tag Free (IL4-H4218) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # AAH67514).

Predicted N-terminus: His 25

蛋白结构(Molecular Characterization)

Online(His 25 - Ser 153) AAH67514

This protein carries no "tag".

The protein has a calculated MW of 14.9 kDa. The protein migrates as 15 and 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS and NaCl. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IL-4, Tag Free (Cat. No. IL4-H4218) SDS-PAGE gel

Human IL-4, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-4, Tag Free (Cat. No. IL4-H4218) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-4 R alpha, His Tag (Cat. No. ILR-H82E9) with a linear range of 2-78 ng/mL (QC tested).

点击申请Protocol

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Human IL-4, Tag Free (Cat. No. IL4-H4218) stimulates the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.27-0.49 ng/ml (Routinely tested).

点击申请Protocol

 

文献引用(Citations)

背景(Background)

Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. Recently, researcher found that the cytokine IL-4 plays a key role in development of innate CD8+ T cells in the thymus of several gene-deficient mouse strains, including Itk, KLF2, CBP and Id3, without previous exposure to antigen.

 

前沿进展

货号/价格

IL4-H4218-20ug / ¥1800

IL4-H4218-50ug / ¥4000

产品定制
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IL-4靶点信息
英文全称:Interleukin-4
中文全称:白细胞介素-4
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床二期
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