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ActiveMax® Recombinant Human IFN-gamma /IFNG

分子别名(Synonym)

Interferon-gamma ,Interferon-γ

表达区间及表达系统(Source)

ActiveMax® Human IFN-gamma, Tag Free (IFG-H4211) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Gln 166 (Accession # AAH70256).

Predicted N-terminus: Gln 24

蛋白结构(Molecular Characterization)

Online(Gln 24 - Gln 166) AAH70256

This protein carries no "tag".

The protein has a calculated MW of 16.8 kDa. The protein migrates as 17 kDa,20 kDa and 24 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

ActiveMax® Human IFN-gamma, Tag Free (Cat. No. IFG-H4211) SDS-PAGE gel

ActiveMax® Human IFN-gamma, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

The bio-activity of ActiveMax® Human IFN-gamma, Tag Free (Cat. No. IFG-H4211) was determined by dose-dependent inhibition of the proliferation of HT-29 cells. The EC50 for this effect is typically 0.41-0.74 ng/mL (Routinely tested).

点击申请Protocol

 

文献引用(Citations)

背景(Background)

Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFNgamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.

 

前沿进展

货号/价格

IFG-H4211-100ug / ¥1900

IFG-H4211-1mg (250ug X 4) / ¥9290

产品定制
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-31604788(上海)
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
IFN-gamma靶点信息
英文全称:Interferon gamma
中文全称:干扰素-γ
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:8详情
最高研发阶段:批准上市
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