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FITC-Labeled Human EGFRvIII Protein, His Tag DMF

分子别名(Synonym)

EGFRvIII

表达区间及表达系统(Source)

FITC-Labeled Human EGFRvIII Protein, His Tag (EGI-HF2H7) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 378 (Accession # NP_001333870.1). It is the FITC labeled form of Human EGFRvIII Protein, His Tag (EGI-H52H4).

Predicted N-terminus: Leu 25

Request for sequence

蛋白结构(Molecular Characterization)

EGFRvIII Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 40.5 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

偶联(Conjugate)

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(Protein Ratio)

The FITC to protein molar ratio is 2-4.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

EGFRvIII SDS-PAGE

FITC-Labeled Human EGFRvIII Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

EGFRvIII ELISA

Immobilized Anti-EGFRvIII Antibody , Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human EGFRvIII Protein, His Tag (Cat. No. EGI-HF2H7) with a linear range of 0.625-5 μg/mL (QC tested).

Protocol

 

背景(Background)

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
The type III EGF deletion mutant receptor (EGFRvIII) is the most common mutation and was first identified in primary human glioblastoma tumors. This tumor-specific antigen is ligand-independent, contains a constitutively active tyrosine kinase domain, and has been shown to be present in a number of human malignancies. EGFRvIII has been selected as a target for CAR-modified T-cell studies in recent years.

 

前沿进展

 
 
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EGFRvIII靶点信息
英文全称:Epidermal growth factor receptor variant III
中文全称:表皮生长因子受体变体III
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:13详情
最高研发阶段:临床二期
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